Inhibition of ACSF2 protects against renal ischemia/reperfusion injury via mediating mitophagy in proximal tubular cells

• Published in: Free radical biology & medicine 2023-03, Vol.198, p.68-82
• Main Authors: Shi, Haoyu, Qi, Hao, Xie, Dongdong, Zhuang, Jiayi, Qi, Huiyue, Dai, Yingbo, Wu, Jiaqing
• Format: Article
• Language: English
• Subjects: Acute kidney injury; Acute Kidney Injury - metabolism; Animals; Ischemia - metabolism; Kidney - metabolism; Mice; Mitochondria function; Mitophagy; Mitophagy - genetics; Renal tubular cell; Reperfusion Injury - metabolism
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2023.02.003

Acute kidney injury (AKI) is a prevalent clinical condition caused by sepsis and ischemia reperfusion (IR) injury. The principal driver of IR-induced AKI involves renal tubular structural changes triggered by the impairment of function in renal tubular cells. The target gene, Acyl-CoA Synthetase Family Member 2 (ACSF2), was retrieved from the GEO database based on high specific expression in renal tubular cells and location in mitochondria. Here, we substantiate that ACSF2 is specifically localized in the mitochondria of the renal tubular epithelium. Functionally silencing ACSF2 in HK2 cells enhanced hypoxia-reoxygenation (HR)-induced mitophagy, restored mitochondrial function and decreased the production of mitochondrial superoxide. Our study demonstrated that these effects were reversed by silencing Bcl-2 19-kDa interacting protein 3 (BNIP3), a receptor regulating mitophagy. In vivo, ACSF2 knockdown significantly enhanced IR-induced mitophagy and improved renal function in mice with IR injury. Conversely, BNIP3 knockdown inhibited mitophagy and exacerbated renal damage in ACSF2-knockdown mice with IR injury. In conclusion, our study demonstrated that inhibition of ACSF2 enhances mitophagy, restoring mitochondrial function and protects against IR-induced AKI, providing a new target and potential strategy for therapy. [Display omitted] •Inhibition of ACSF2 protects against mice renal ischemia and reperfusion injury.•Knockdown of ACSF2 enhances mitophagy and restores mitochondrial function in HK-2 cells with HR-treated.•Knockdown of ACSF2 reduces mitochondrial superoxide production and lipid peroxidation in HK-2 cells with HR-treated.•Inhibition of ACSF2 expression enhances mitophagy by promoting BNIP3 binding to mitochondria.