Exogenous application of Bradyrhizobium japonicum AC20 enhances soybean tolerance to atrazine via regulating rhizosphere soil microbial community and amino acid, carbohydrate metabolism related genes expression

• Published in: Plant physiology and biochemistry 2023-03, Vol.196, p.472-483
• Main Authors: Jiang, Duo, Li, Yu, Wang, Jianmin, Lv, Xinyu, Jiang, Zhao, Cao, Bo, Qu, Jianhua, Ma, Shouyi, Zhang, Ying
• Format: Article
• Language: English
• Subjects: Amino Acids; Atrazine; Atrazine - pharmacology; Bradyrhizobium - genetics; Carbohydrate Metabolism; C–N metabolism; Glycine max; Microbiota; Nitrogen; Rhizobium; Rhizosphere; RNA, Ribosomal, 16S; Seedlings - genetics; Soil; Soybean; Symbiosis; Transcriptomic
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2023.02.007

Atrazine is used to control broad-leaved weeds in farmland and has negative impacts on soybean growth. Legume-rhizobium symbiosis plays an important role in regulating abiotic stress tolerance of plants, however, the mechanisms of rhizobia regulate the tolerance of soybean to atrazine based on the biochemical responses of the plant-soil system are limited. In this experiment, Glycine max (L.) Merr. Dongnong 252, planted in 20 mg kg−1 of atrazine-contaminated soil, was inoculated with Bradyrhizobium japonicum AC20, and the plant growth, rhizosphere soil microbial diversity and the expression of the genes related to soybean carbon and nitrogen metabolism were assessed. The results indicated that strain AC20 inoculation alleviated atrazine-induced growth inhibition via increasing the contents of leghemoglobin and total nitrogen in soybean seedlings. The psbA gene expression level of the soybean seedlings that inoculated strain AC20 was 1.4 times than that of no rhizobium inoculating treatments. Moreover, the inoculated AC20 increased the abundance of Acidobacteria and Actinobacteria in soybean rhizosphere. Transcriptome analysis demonstrated that strain AC20 regulated the genes expression of amino acid metabolism and carbohydrate metabolism of soybean seedlings. Correlation analysis between 16S rRNA and transcriptome showed that strain AC20 reduced Planctomycetes abundance so as to down-regulated the expression of genes Glyma. 13G087800, Glyma. 12G005100 and Glyma.12G098900 involved in starch synthesis pathway of soybean leaves. These results provide available information for the rhizobia application to enhance the atrazine tolerate in soybean seedlings. [Display omitted] •Rhizobium AC20 greatly relieved the atrazine induced loss of nitrogen in soybean.•AC20 can regulate the structure of microbial community in soybean rhizosphere soil.•AC20 regulated amino acid, carbohydrate metabolism related genes expression in soybean.•AC20 can abate the toxic effect of atrazine to improve soybean seedling growth.•Rhizosphere Planctomycetes promotes sucrose decomposition and TCA cycle in soybean.