Metabolomic approach of azole fungicides in radish (Raphanus sativus): Perspective of functional metabolites

Azole fungicides is one of the major fungicides in agricultural field. In this study, toxic effects of diniconazole (DIN), metconazole (MET), and tebuconazole (TEB) to radish leaves and roots were investigated using targeted metabolomics with gas chromatography-mass spectrometry (GC-MS/MS). Especial...

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Bibliographic Details
Published in:Journal of hazardous materials 2023-04, Vol.448, p.130937-130937, Article 130937
Main Authors: Yu, Ji-Woo, Song, Min-Ho, Keum, Young-Soo, Lee, Ji-Ho
Format: Article
Language:English
Subjects:
Azole
Azoles - metabolism
Azoles - toxicity
Fungicides, Industrial - metabolism
Glucosinolates
Glucosinolates - chemistry
Glucosinolates - metabolism
Glucosinolates - pharmacology
Metabolomics
Metabolomics - methods
Phytosterols
Radish
Raphanus - chemistry
Raphanus - metabolism
Tandem Mass Spectrometry
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Summary:Azole fungicides is one of the major fungicides in agricultural field. In this study, toxic effects of diniconazole (DIN), metconazole (MET), and tebuconazole (TEB) to radish leaves and roots were investigated using targeted metabolomics with gas chromatography-mass spectrometry (GC-MS/MS). Especially, the changes of functional chemicals, including phytosterols and glucosinolates evaluated. Radish leaves and roots were harvested after 7 days and 14 days from last exposure. In multivariate analysis, the experimental groups showed clear separation in PCA and PLS-DA score plots. Phytosterols and glucosinolates were significantly changed by azole fungicide. Six metabolic pathways which are affected by fungicides were selected and showed similar patterns regardless of the type of azole fungicide used. As a result, azole fungicide induces the defense mechanisms of plants and affects both primary and secondary metabolism. [Display omitted] •Azole fungicides induce metabolic changes in radish leaves and roots.•Primary metabolites tend to be decreased in leaves while they were increased in roots.•Phytosterol contents were changed by azole application.•Glucosinolates which relate to defense mechanism were increased.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2023.130937