Molecular characterization of horse nettle virus A, a new member of subgroup B of the genus Nepovirus

• Published in: Archives of virology 2023-03, Vol.168 (3), p.86-86, Article 86
• Main Authors: Zhou, Jing, Hu, Xiaojun, Vieira, Paulo, Atha, Benjamin, McFarland, Clint, Foster, Joseph A., Hurtado-Gonzales, Oscar P.
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; Amino Acid Sequence; Amino acids; Annotated Sequence Record; Biomedical and Life Sciences; Biomedicine; Genome, Viral; Genomes; Infectious Diseases; Laboratories; Medical Microbiology; Nepovirus; Nepovirus - genetics; New species; Next-generation sequencing; Nucleotides; Phylogenetics; Phylogeny; Polyadenylation; Proteins; Recombination; RNA viruses; RNA, Viral - chemistry; RNA, Viral - genetics; Solanum; Virology
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-023-05708-7

A new positive-strand RNA virus was discovered in a horse nettle plant, using high-throughput sequencing (HTS), and its complete genome, consisting of RNA1 and RNA2, which are 7522 and 4710 nucleotides in length, respectively, was characterized. Each genome segment contains a single open reading frame flanked by 5′ and 3′ untranslated regions (UTRs), followed by a poly(A) tail at the 3′ end. The encoded proteins have the highest amino acid sequence identity (55% and 45%) to the polyprotein encoded by RNA1 of tomato black ring virus (TBRV) and RNA2 of potato virus B (PVB), respectively. Its genome organization and phylogenetic relationship to other nepoviruses suggested that this virus is a novel member of subgroup B, and recombination analysis revealed its evolutionary history within the subgroup. These results suggest the new virus, provisionally named "horse nettle virus A", represents a new species within the genus Nepovirus.