Magnaporthe oryzae endoplasmic reticulum membrane complex regulates the biogenesis of membrane proteins for pathogenicity

• Published in: The New phytologist 2023-05, Vol.238 (3), p.1163-1181
• Main Authors: Liu, Ning, Huang, Manna, Liang, Xinyuan, Cao, Miao, Lun, Zhiqin, Zhang, Yan, Yang, Jun, Bhadauria, Vijai, Zhao, Wensheng, Yan, Jiye, Peng, You‐Liang, Lu, Xunli
• Format: Article
• Language: English
• Subjects: Atg9; Autophagy; Biogenesis; Biosynthesis; cell wall integrity; Cell walls; Chs4; Endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Eukaryotes; Fks1; Fungal Proteins - metabolism; Fungi; Fungicides; Integral membrane proteins; Magnaporthe; Magnaporthe oryzae; Magnesium; Magnesium - metabolism; magnesium transport; Membrane Proteins - genetics; Membrane Proteins - metabolism; Membranes; Mnr2; MoEmc5; Mutants; Oryza - metabolism; Pathogenicity; Pathogens; Phytopathogenic fungi; Plant Diseases - microbiology; Proteins; Virulence
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.18810

Summary In eukaryotes, the majority of newly synthesized integral membrane proteins are inserted into the endoplasmic reticulum (ER) membrane before transferred to their functional sites. The conserved ER membrane complex (EMC) takes part in the insertion process for tail‐anchored membrane proteins. However, the function of EMC in phytopathogenic fungi has not been characterized. Here, we report the identification and functional characterization of two EMC subunits MoEmc5 and MoEmc2 in Magnaporthe oryzae. The knockout mutants ΔMoemc5 and ΔMoemc2 exhibit substantial defect in autophagy, pathogenicity, cell wall integrity, and magnesium ion sensitivity. We demonstrate that the autophagy process was severely impaired in the ΔMoemc5 and ΔMoemc2 mutants because of the low‐protein steady‐state level of Atg9, the sole membrane‐associated autophagy protein. Furthermore, the protein level of membrane proteins Chs4, Fks1, and MoMnr2 is also significantly reduced in the ΔMoemc5 and ΔMoemc2 strains, leading to their supersensitivity to Calcofluor white, Congo red, and magnesium. In addition, MoEmc5, but not MoEmc2, acts as a magnesium transporter independent of its EMC function. Magnaporthe oryzae EMC regulates the biogenesis of membrane proteins for autophagy and virulence; therefore, EMC subunits could be potential targets for fungicide design in the future.