Comparison of the anti-cancer activity of 5-aminolevulinic acid-mediated photodynamic therapy after continuous wave and pulse irradiation in different histological types of canine mammary sarcoma tumors

Canine mammary sarcoma tumors (CMST) are the most aggressive tumors with poor prognosis in dogs. Due to inadequate treatment options for CMST, recent studies have focused on alternative treatment strategies. We previously determined the optimized protocol of 5-ALA-based photodynamic therapy (PDT) in...

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Bibliographic Details
Published in:Lasers in medical science 2023-02, Vol.38 (1), p.70-70, Article 70
Main Authors: Turna, Ozge, Deveci Ozkan, Asuman, Guney Eskiler, Gamze, Baykal, Aslihan, Ozten, Ozge, Yildirim, Funda, Armutak, Elif Ilkay, Kamanli, Ali Furkan, Lim, Hyun Soo, Kaleli, Suleyman, Kasikci, Guven, Yildiz, Salih Zeki
Format: Article
Language:English
Subjects:
Aminolevulinic acid
Aminolevulinic Acid - pharmacology
Aminolevulinic Acid - therapeutic use
Animals
Annexin V
Anticancer properties
Antitumor activity
Apoptosis
Apoptosis - radiation effects
Breast cancer
Caspase-3
Caspase-9
Cell Line, Tumor
Cell proliferation
Chondrosarcoma
Continuous radiation
Dentistry
Dogs
In vivo methods and tests
Iodides
Irradiation
Lasers
Liposarcoma
Liposarcoma - drug therapy
Liposarcoma - genetics
Liposarcoma - radiotherapy
Medicine
Medicine & Public Health
Molecular modelling
mRNA
Optical Devices
Optics
Original Article
Osteosarcoma
Photochemotherapy - methods
Photodynamic therapy
Photonics
Photosensitizing Agents - pharmacology
Photosensitizing Agents - therapeutic use
Propidium iodide
Quantum Optics
Radiation
Reactive Oxygen Species - metabolism
Sarcoma
Tumors
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Summary:Canine mammary sarcoma tumors (CMST) are the most aggressive tumors with poor prognosis in dogs. Due to inadequate treatment options for CMST, recent studies have focused on alternative treatment strategies. We previously determined the optimized protocol of 5-ALA-based photodynamic therapy (PDT) in canine liposarcoma. However, its molecular mechanisms in the treatment of different histological types of CMST remain unclear. In this context, we, for the first time, assessed 5-aminolevulinic acid (5-ALA)-PDT–mediated anti-cancer activity and its molecular mechanism after continuous wave (CW) and pulse radiation (PR) on three different histological types (liposarcoma, chondrosarcoma, and osteosarcoma) of CMST cells by WST-1, Annexin V, ROS, acridine orange/propidium iodide staining, RT-PCR, and western blot analysis. Our findings showed that 5-ALA/PDT significantly suppressed the proliferation of CMST cells ( p  
ISSN:1435-604X
0268-8921
1435-604X
DOI:10.1007/s10103-023-03732-2