Impact of Alpinia galanga and zinc on semen quality and some reproductive hormone constituents in California rabbit bucks

• Published in: Zygote (Cambridge) 2023-04, Vol.31 (2), p.188-194
• Main Authors: El-Speiy, M E, El-Sawy, M A, Sadaka, T A, Abd-Elaal, M A, Habib, M R, Abdella, M M, Khattab, Mostafa S A
• Format: Article
• Language: English
• Subjects: Acids; Alpinia; Alpinia galanga; Analytical chemistry; Animals; Aquatic plants; Artificial insemination; Body weight; Carotenoids; Cholesterol; Density; Diet; Drinking water; Drug dosages; Dry matter; Estrogens; Fertility; Flavonoids; Follicle Stimulating Hormone; Gonadotropins; High density lipoprotein; Hormones; Infertility; Litter size; Luteinizing Hormone; Male; Males; Motility; Phenols; Pituitary (anterior); Plant Preparations - pharmacology; Progesterone; Rabbits; Rhizomes; Seeds; Semen; Semen Analysis; Sperm; Sperm Motility; Spermatozoa; Synergism; Testosterone; Triglycerides; Zinc; Zinc - pharmacology; Zinc sulfate
• ISSN: 0967-1994; 1469-8730
• DOI: 10.1017/S0967199423000011

The objective of the current study was to investigate the influence of synergism of the dry powder of rhizomes (AGR) and/or zinc sulfate in the diet on semen quality and reproductive traits of California rabbit bucks. The study was conducted in two stages. First stage: appreciation of semen characteristics, 36 California rabbit bucks (aged 5 months) with average body weights of 2980 g were divided randomly into six treatments (six individuals each). The treatment groups were: first group, control fed basal diet (C); second group, fed basal diet plus 1 g AGR/kg dry matter (DM) (AGR1); third group, fed basal diet plus 2 g AGR/kg DM (AGR2); fourth group, fed basal diet plus 200 mg Zn/litre drinking water (Zn); fifth group, fed basal diet plus 1 g AGR/kg DM and 200 mg Zn/litre drinking water (AGR1 + Zn); sixth group, fed basal diet plus 2 g AGR/kg DM and 200 mg Zn/litre drinking water (AGR2 + Zn). Second stage: the previous bucks were used to determine the efficiency of semen on reproductive fertility traits, 48 mature does (aged 6 months, nulliparous) with an average body weight of 3050 ± 20.7 g were divided randomly into six treatments and inseminated with previous groups of treated bucks. The results of the first stage, recorded high activity on gonadotropins hormones: follicle-stimulating hormone (FSH) and luteinizing hormone (LH), free testosterone (FT), progesterone (P ) and oestrogen (E 17β) concentrations for AGR1 + Zn and AGR2 + Zn compared with the control group. Groups AGR1, AGR2, AGR1 + Zn and AGR2 + Zn had significantly lowered concentrations of triglycerides, total cholesterol, low-density lipoprotein, and malondialdehyde (MDA), whereas high-density lipoprotein and total antioxidant capacity (TAC) were increased significantly compared with the control group. The group supplemented with AGR with or without Zn had significantly improved ejaculate volume, advanced motility, sperm concentration, and cell integrity. Fertility rate and litter size were improved in all groups compared with the control. It was concluded that supplementing diets with and Zn significantly increased sperm percentage, motility and reproductive hormones (testosterone, FSH, LH, E 17β, P ). This suggested that this plant when used may be favourable for improved sperm quality and fertility parameters.