A structure defined pectin SA02B from Semiaquilegia adoxoides is metabolized by human gut microbes

Polysaccharide is one of the major factors for shaping the gut microbiota. However, bioactivity of polysaccharide isolated from Semiaquilegia adoxoides on human gut microbiota remains unclear. Thus, we hypothesize gut microbes may act on it. Herein, pectin SA02B from the roots of Semiaquilegia adoxo...

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Published in:International journal of biological macromolecules 2023-04, Vol.234, p.123673-123673, Article 123673
Main Authors: Li, Saijuan, Li, Tingting, Wang, Binqiang, Wen, Chang, Li, Meixia, Ding, Kan
Format: Article
Language:English
Subjects:
Degradation
Gastrointestinal Microbiome
Human gut microbiota
Humans
Pectin
Pectins - chemistry
Plant Roots - chemistry
Polysaccharide
Polysaccharides - chemistry
Semiaquilegia
Semiaquilegia adoxoides
Short chain fatty acids
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Summary:Polysaccharide is one of the major factors for shaping the gut microbiota. However, bioactivity of polysaccharide isolated from Semiaquilegia adoxoides on human gut microbiota remains unclear. Thus, we hypothesize gut microbes may act on it. Herein, pectin SA02B from the roots of Semiaquilegia adoxoides with molecular weight 69.26 kDa was elucidated. The backbone of SA02B was composed of alternate 1, 2-linked α-Rhap and 1, 4-linked α-GalpA, with branches of terminal (T) -, 1, 4-, 1, 3- and 1, 3, 6-linked β-Galp, T-, 1, 5- and 1, 3, 5-linked α-Araf and T-, 1, 4-linked-β-Xylp substituted at C-4 of 1, 2, 4-linked α-Rhap. Bioactivity screening showed SA02B promoted the growth of Bacteroides spp. which deconstructed it into monosaccharide. Simultaneously, we observed competition might exist between Bacteroides spp. and probiotics. Besides, we found that both Bacteroides spp. and probiotics could generate SCFAs grown on SA02B. Our findings highlight SA02B may deserve as a prebiotic to be explored to benefit the health gut microbiota. •A special pectin SA02B was isolated and purified from Semiaquilegia adoxoides.•Structure of SA02B contained RG-I backbone, and branches with Gal, Ara and Xyl.•SA02B promoted the growth of Bacteroides spp. as the sole carbon source.•Competition might exist between Bacteroides spp. and probiotics.•SA02B was metabolized by human gut microbiota to generate SCFAs.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.123673