Molecular structure, IgE binding capacity and gut microbiota of ovalbumin conjugated to fructose and galactose:A comparative study

Ovalbumin (OVA) was modified by fructose (Fru) and galactose (Gal) to study the structure, IgG/IgE binding capacity and effects on human intestinal microbiota of the conjugated products. Compared with OVA-Fru, OVA-Gal has a lower IgG/IgE binding capacity. The reduction of OVA is not only associated...

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Bibliographic Details
Published in:International journal of biological macromolecules 2023-04, Vol.234, p.123640-123640, Article 123640
Main Authors: Mao, Ji-hua, Zhang, Kai, He, Ying-fei, Liu, Jun, Shao, Yan-hong, Tu, Zong-cai
Format: Article
Language:English
Subjects:
Fructose
Galactose
Gastrointestinal Microbiome
Glycation
Gut microbiota
Humans
IgG/IgE binding capacity
Immunoglobulin E - metabolism
Immunoglobulin G - chemistry
Molecular Structure
Ovalbumin
Ovalbumin - chemistry
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Summary:Ovalbumin (OVA) was modified by fructose (Fru) and galactose (Gal) to study the structure, IgG/IgE binding capacity and effects on human intestinal microbiota of the conjugated products. Compared with OVA-Fru, OVA-Gal has a lower IgG/IgE binding capacity. The reduction of OVA is not only associated with the glycation of R84, K92, K206, K263, K322 and R381 in the linear epitopes, but also with conformational epitope changes, manifested as secondary and tertiary structural changes caused by Gal glycation. In addition, OVA-Gal could alter the structure and abundance of gut microbiota at phylum, family, and genus levels and restore the abundance of bacteria associated with allergenicity, such as Barnesiella, Christensenellaceae_R-7_group, and Collinsela, thereby reducing allergic reactions. These results indicate that OVA-Gal glycation can reduce the IgE binding capacity of OVA and change the structure of human intestinal microbiota. Therefore, Gal glycation may be a potential method to reduce protein allergenicity. [Display omitted]
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.123640