High bioethanol titer and yield from phosphoric acid plus hydrogen peroxide pretreated paper mulberry wood through optimization of simultaneous saccharification and fermentation

• Published in: Bioresource technology 2023-04, Vol.374, p.128759-128759, Article 128759
• Main Authors: Ajayo, Pleasure Chisom, Wang, Qing, Huang, Mei, Zhao, Li, Tian, Dong, He, Jinsong, Fang, Dexin, Hu, Jinguang, Shen, Fei
• Format: Article
• Language: English
• Subjects: Cellulosic ethanol; Ethanol; Fermentation; Hydrogen Peroxide; Hydrolysis; Isothermal SSF; Morus; Process optimization; Response surface methodology; Saccharomyces cerevisiae; Wood; Woody biomass pretreatment
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.128759

[Display omitted] •Key SSF variables were optimized for PHP-pretreated PM wood at two thermal levels.•SSF process at 35 °C is preferable compared to a higher trade-off level of 38 °C.•With optimal SSF at 35 °C, high ethanol titer (77 g/L) and yield (84%) were gained.•Optimal SSF at 35 °C can reduce energy demand while increasing ethanol production. The optimization of key simultaneous saccharification and fermentation (SSF) parameters for bioethanol production from phosphoric acid plus hydrogen peroxide pretreated paper mulberry wood was carried out under two isothermal scenarios; the yeast optimum and trade-off temperatures of 35 and 38 °C, respectively. The optimal conditions established for SSF at 35 °C (solid loading: 16%; enzyme dosage: 9.8 mg protein/g glucan; and yeast concentration: 6.5 g/L) achieved high ethanol titer and yield of 77.34 g/L and 84.60% (0.432 g/g), respectively. These corresponded to 1.2 and 1.3-folds increases, compared to the results of the optimal SSF at a relatively higher temperature of 38 °C. The information from this study would prove beneficial in reducing process energy demands to some extent, while also helping to achieve high levels of both ethanol concentration and yield that are desired in cellulosic ethanol production.