Exosomes rich in Wnt5 improved circadian rhythm dysfunction via enhanced PPARγ activity in the 6-hydroxydopamine model of Parkinson's disease

• Published in: Neuroscience letters 2023-04, Vol.802, p.137139-137139, Article 137139
• Main Authors: Li, Zongshan, Li, Yongang, Xu, Xiaomin, Gu, Jiachen, Chen, Huimin, Gui, Yaxing
• Format: Article
• Language: English
• Subjects: Animals; Circadian Rhythm; Exosomes - metabolism; MSC-Exosomes; Oxidopamine - metabolism; Parkinson Disease - metabolism; Parkinson Disease - therapy; PPAR gamma - metabolism; PPARγ; Rats; Sleep disorder; Wnt Proteins - metabolism
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2023.137139

•Sleep disorder was improved in PD rat treated with BMSC derived exosomes.•Striatal 5-HT and dopamine levels were increased in PD model treated with BMSC-EXO.•Rescued expression of circadian rhythm associated genes in PD rat treated with BMSC derived exosomes.•PPARγ expression was induced in PD rat treated with BMSC derived exosomes.•BMSC derived exosomes rescued OHDA induced ΔΨm collapse in neuronal cells. Sleep disorder is one of the most common non-motor symptoms in Parkinson's disease (PD) and even appear as early symptoms. Here we investigated the therapeutic potential of mesenchymal stem cell-derived exosomes (MSC-EXOs) on sleep disorder in PD rats. 6-hydroxydopa (6-OHDA) was used to establish the PD rat model. BMSCquiescent-EXO and BMSCinduced-EXO groups were given intravenous injection 100 µg/g per day for 4 weeks, while control groups were given intravenous injection of the same volume of normal saline. The total sleep time, slow-wave sleep time and fast-wave sleep time in the BMSCquiescent-EXO and BMSCinduced-EXO groups were significantly prolonged (P