Specific nanoarchitecture of silica nanoparticles codoped with the oppositely charged Mn2+ and Ru2+ complexes for dual paramagnetic-luminescent contrasting effects

• Published in: Nanomedicine 2023-04, Vol.49, p.102665-102665, Article 102665
• Main Authors: Fedorenko, Svetlana, Stepanov, Alexey, Bochkova, Olga, Kholin, Kirill, Nizameev, Irek, Voloshina, Alexandra, Tyapkina, Oksana, Samigullin, Dmitry, Kleshnina, Sofiya, Akhmadeev, Bulat, Romashchenko, Alexander, Zavjalov, Evgenii, Amirov, Rustem, Mustafina, Asiya
• Format: Article
• Language: English
• Subjects: Contrast agents; Ex vivo biodistribution; Manganese complexes; Relaxivity; Silica nanoparticles
• ISSN: 1549-9634; 1549-9642
• DOI: 10.1016/j.nano.2023.102665

The silica nanoparticles (SNs) co-doped with paramagnetic ([Mn(HL)]n-,) and luminescent ([Ru(dipy)3]2+) complexes are represented. The specific distribution of [Mn(HL)]n- within the SNs allows to achieve about ten-fold enhancing in magnetic relaxivities in comparison with those of [Mn(HL)]n- in solutions. The leaching of [Mn(HL)]n- from the shell can be minimized through the co-doping of [Ru(dipy)3]2+ into the core of the SNs. The co-doped SNs exhibit colloid stability in aqueous solutions, including those modeling a blood serum. The surface of the co-doped SNs was also decorated by amino- and carboxy-groups. The cytotoxicity, hemoagglutination and hemolytic activities of the co-doped SNs are on the levels convenient for “in vivo” studies, although the amino-decorated SNs cause more noticeable agglutination and suppression of cell viability. The co-doped SNs being intravenously injected into mice allows to reveal their biodistribution in both ex vivo and in vivo conditions through confocal microscopy and magnetic resonance imaging correspondingly. Codoping of the oppositely charged paramagnetic and luminescent complexes into silica nanoparticles produces dual contrasting agents applicable in both magnetic resonance imaging and confocal microscopy. Surface decoration of the nanoparticles affects their cytotoxicity, hemocompatibility and distribution within the mice after their intravenous injection by the nanoparticles. The latter is revealed by the ex vivo confocal analysis of the tissue samples harvested from the mice. [Display omitted]