Impact of different brushing/abrasion protocols on erosive tooth wear for in vitro studies

To investigate the influence of different toothbrushing (with dentifrice) protocols on the progression of erosive tooth wear for in vitro studies. Bovine enamel specimens were randomly distributed into 12 experimental groups (n = 10), according to the study factors: (1) brushing movement (horizontal...

Full description

Saved in:
Bibliographic Details
Published in:Archives of oral biology 2023-04, Vol.148, p.105657-105657, Article 105657
Main Authors: Sakae, Letícia Oba, Renzo, Ana Luísa Meira, Viana, Ítallo Emídio Lira, Niemeyer, Samira Helena, Carvalho, Thiago Saads, Scaramucci, Taís
Format: Article
Language:English
Subjects:
Animals
Cattle
Enamel
Erosive tooth wear
Saliva, Artificial
Study design
Surface loss
Tooth Abrasion
Tooth Erosion
Tooth Wear
Toothbrushing
Toothpastes
Water
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c377t-85a8521a1753845d7860ec13c0cfd6b81f725852e16ab911eefa24911f9f303e3
cites cdi_FETCH-LOGICAL-c377t-85a8521a1753845d7860ec13c0cfd6b81f725852e16ab911eefa24911f9f303e3
container_end_page 105657
container_issue
container_start_page 105657
container_title Archives of oral biology
container_volume 148
creator Sakae, Letícia Oba
Renzo, Ana Luísa Meira
Viana, Ítallo Emídio Lira
Niemeyer, Samira Helena
Carvalho, Thiago Saads
Scaramucci, Taís
description To investigate the influence of different toothbrushing (with dentifrice) protocols on the progression of erosive tooth wear for in vitro studies. Bovine enamel specimens were randomly distributed into 12 experimental groups (n = 10), according to the study factors: (1) brushing movement (horizontal or circular); (2) slurry diluent (artificial saliva or distilled water); (3) toothpaste dilution ratio (1:2, 1:3 or 1:4). A 5-day erosion-abrasion cycling model was performed, each consisting of 4 erosive challenges (0.3 % citric acid, pH=2.6) followed by 60 min exposure to artificial saliva. Brushing with fluoride toothpaste (15 s, 1400 ppm F-, AmF) was carried out 2x/day. Enamel surface loss (SL) was determined by optical profilometry. Data were statistically analyzed with three-way ANOVA and Tukey tests (α = 0.05). SL was lower for the horizontal movement than for the circular (p = 0.044). There were no significant differences among the dilution ratios for artificial saliva. For distilled water, the more concentrated slurry (1:2) presented greater surface loss than the less concentrated slurries (1:3 and 1:4, p = 0.049 and p = 0.014, respectively). Dilutions with artificial saliva at ratios 1:3 and 1:4 presented higher surface loss than with distilled water (p = 0.008 and p 
doi_str_mv 10.1016/j.archoralbio.2023.105657
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2780075936</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003996923000456</els_id><sourcerecordid>2780075936</sourcerecordid><originalsourceid>FETCH-LOGICAL-c377t-85a8521a1753845d7860ec13c0cfd6b81f725852e16ab911eefa24911f9f303e3</originalsourceid><addsrcrecordid>eNqNkEtPAyEQgInR2Pr4CwZvXrbCIrAcTeOjiYkXvXghLDtYmu1Sga3x34upGo-emJl8w8x8CJ1TMqOEisvVzES7DNH0rQ-zmtSs1Lngcg9NaSNVRTkR-2hKCGGVUkJN0FFKq5JyIeghmjDR1FLVaopeFuuNsRkHhzvvHEQYMm7jmJZ-eL00bTTJhwFvYsjBhj7hkkAMyW8B5xDyEr-DidiFiP2Atz7HgFMeOw_pBB040yc4_X6P0fPtzdP8vnp4vFvMrx8qy6TMVcNNw2tqqOSsueKdbAQBS5kl1nWibaiTNS8EUGFaRSmAM_VVCZxyjDBgx-hi929Z8m2ElPXaJwt9bwYIY9K1bAiRXDFRULVDbTkhRXB6E_3axA9Nif5Sq1f6j1r9pVbv1Jbes-8xY7uG7rfzx2UB5jsAyrFbD1En62Gw0PkINusu-H-M-QR0_JEB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2780075936</pqid></control><display><type>article</type><title>Impact of different brushing/abrasion protocols on erosive tooth wear for in vitro studies</title><source>ScienceDirect Freedom Collection</source><creator>Sakae, Letícia Oba ; Renzo, Ana Luísa Meira ; Viana, Ítallo Emídio Lira ; Niemeyer, Samira Helena ; Carvalho, Thiago Saads ; Scaramucci, Taís</creator><creatorcontrib>Sakae, Letícia Oba ; Renzo, Ana Luísa Meira ; Viana, Ítallo Emídio Lira ; Niemeyer, Samira Helena ; Carvalho, Thiago Saads ; Scaramucci, Taís</creatorcontrib><description>To investigate the influence of different toothbrushing (with dentifrice) protocols on the progression of erosive tooth wear for in vitro studies. Bovine enamel specimens were randomly distributed into 12 experimental groups (n = 10), according to the study factors: (1) brushing movement (horizontal or circular); (2) slurry diluent (artificial saliva or distilled water); (3) toothpaste dilution ratio (1:2, 1:3 or 1:4). A 5-day erosion-abrasion cycling model was performed, each consisting of 4 erosive challenges (0.3 % citric acid, pH=2.6) followed by 60 min exposure to artificial saliva. Brushing with fluoride toothpaste (15 s, 1400 ppm F-, AmF) was carried out 2x/day. Enamel surface loss (SL) was determined by optical profilometry. Data were statistically analyzed with three-way ANOVA and Tukey tests (α = 0.05). SL was lower for the horizontal movement than for the circular (p = 0.044). There were no significant differences among the dilution ratios for artificial saliva. For distilled water, the more concentrated slurry (1:2) presented greater surface loss than the less concentrated slurries (1:3 and 1:4, p = 0.049 and p = 0.014, respectively). Dilutions with artificial saliva at ratios 1:3 and 1:4 presented higher surface loss than with distilled water (p = 0.008 and p &lt; 0.001, respectively); however, for 1:2 ratio, there were no significant differences between the diluents. The in vitro progression of enamel SL was influenced by the brushing movement, as well as the combination of the dilution ratio and the diluent of the toothpaste slurry, and therefore, all these factors must be considered when comparing results from different studies. •Designing the brushing protocol of ETW studies is complex and challenging.•ETW is influenced by the brushing movement, slurry diluent and dilution ratio.•Standardization of ETW protocols is desirable to allow comparison between studies.</description><identifier>ISSN: 0003-9969</identifier><identifier>EISSN: 1879-1506</identifier><identifier>DOI: 10.1016/j.archoralbio.2023.105657</identifier><identifier>PMID: 36827929</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Cattle ; Enamel ; Erosive tooth wear ; Saliva, Artificial ; Study design ; Surface loss ; Tooth Abrasion ; Tooth Erosion ; Tooth Wear ; Toothbrushing ; Toothpastes ; Water</subject><ispartof>Archives of oral biology, 2023-04, Vol.148, p.105657-105657, Article 105657</ispartof><rights>2023 Elsevier Ltd</rights><rights>Copyright © 2023 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-85a8521a1753845d7860ec13c0cfd6b81f725852e16ab911eefa24911f9f303e3</citedby><cites>FETCH-LOGICAL-c377t-85a8521a1753845d7860ec13c0cfd6b81f725852e16ab911eefa24911f9f303e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36827929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakae, Letícia Oba</creatorcontrib><creatorcontrib>Renzo, Ana Luísa Meira</creatorcontrib><creatorcontrib>Viana, Ítallo Emídio Lira</creatorcontrib><creatorcontrib>Niemeyer, Samira Helena</creatorcontrib><creatorcontrib>Carvalho, Thiago Saads</creatorcontrib><creatorcontrib>Scaramucci, Taís</creatorcontrib><title>Impact of different brushing/abrasion protocols on erosive tooth wear for in vitro studies</title><title>Archives of oral biology</title><addtitle>Arch Oral Biol</addtitle><description>To investigate the influence of different toothbrushing (with dentifrice) protocols on the progression of erosive tooth wear for in vitro studies. Bovine enamel specimens were randomly distributed into 12 experimental groups (n = 10), according to the study factors: (1) brushing movement (horizontal or circular); (2) slurry diluent (artificial saliva or distilled water); (3) toothpaste dilution ratio (1:2, 1:3 or 1:4). A 5-day erosion-abrasion cycling model was performed, each consisting of 4 erosive challenges (0.3 % citric acid, pH=2.6) followed by 60 min exposure to artificial saliva. Brushing with fluoride toothpaste (15 s, 1400 ppm F-, AmF) was carried out 2x/day. Enamel surface loss (SL) was determined by optical profilometry. Data were statistically analyzed with three-way ANOVA and Tukey tests (α = 0.05). SL was lower for the horizontal movement than for the circular (p = 0.044). There were no significant differences among the dilution ratios for artificial saliva. For distilled water, the more concentrated slurry (1:2) presented greater surface loss than the less concentrated slurries (1:3 and 1:4, p = 0.049 and p = 0.014, respectively). Dilutions with artificial saliva at ratios 1:3 and 1:4 presented higher surface loss than with distilled water (p = 0.008 and p &lt; 0.001, respectively); however, for 1:2 ratio, there were no significant differences between the diluents. The in vitro progression of enamel SL was influenced by the brushing movement, as well as the combination of the dilution ratio and the diluent of the toothpaste slurry, and therefore, all these factors must be considered when comparing results from different studies. •Designing the brushing protocol of ETW studies is complex and challenging.•ETW is influenced by the brushing movement, slurry diluent and dilution ratio.•Standardization of ETW protocols is desirable to allow comparison between studies.</description><subject>Animals</subject><subject>Cattle</subject><subject>Enamel</subject><subject>Erosive tooth wear</subject><subject>Saliva, Artificial</subject><subject>Study design</subject><subject>Surface loss</subject><subject>Tooth Abrasion</subject><subject>Tooth Erosion</subject><subject>Tooth Wear</subject><subject>Toothbrushing</subject><subject>Toothpastes</subject><subject>Water</subject><issn>0003-9969</issn><issn>1879-1506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqNkEtPAyEQgInR2Pr4CwZvXrbCIrAcTeOjiYkXvXghLDtYmu1Sga3x34upGo-emJl8w8x8CJ1TMqOEisvVzES7DNH0rQ-zmtSs1Lngcg9NaSNVRTkR-2hKCGGVUkJN0FFKq5JyIeghmjDR1FLVaopeFuuNsRkHhzvvHEQYMm7jmJZ-eL00bTTJhwFvYsjBhj7hkkAMyW8B5xDyEr-DidiFiP2Atz7HgFMeOw_pBB040yc4_X6P0fPtzdP8vnp4vFvMrx8qy6TMVcNNw2tqqOSsueKdbAQBS5kl1nWibaiTNS8EUGFaRSmAM_VVCZxyjDBgx-hi929Z8m2ElPXaJwt9bwYIY9K1bAiRXDFRULVDbTkhRXB6E_3axA9Nif5Sq1f6j1r9pVbv1Jbes-8xY7uG7rfzx2UB5jsAyrFbD1En62Gw0PkINusu-H-M-QR0_JEB</recordid><startdate>202304</startdate><enddate>202304</enddate><creator>Sakae, Letícia Oba</creator><creator>Renzo, Ana Luísa Meira</creator><creator>Viana, Ítallo Emídio Lira</creator><creator>Niemeyer, Samira Helena</creator><creator>Carvalho, Thiago Saads</creator><creator>Scaramucci, Taís</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202304</creationdate><title>Impact of different brushing/abrasion protocols on erosive tooth wear for in vitro studies</title><author>Sakae, Letícia Oba ; Renzo, Ana Luísa Meira ; Viana, Ítallo Emídio Lira ; Niemeyer, Samira Helena ; Carvalho, Thiago Saads ; Scaramucci, Taís</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-85a8521a1753845d7860ec13c0cfd6b81f725852e16ab911eefa24911f9f303e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Enamel</topic><topic>Erosive tooth wear</topic><topic>Saliva, Artificial</topic><topic>Study design</topic><topic>Surface loss</topic><topic>Tooth Abrasion</topic><topic>Tooth Erosion</topic><topic>Tooth Wear</topic><topic>Toothbrushing</topic><topic>Toothpastes</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakae, Letícia Oba</creatorcontrib><creatorcontrib>Renzo, Ana Luísa Meira</creatorcontrib><creatorcontrib>Viana, Ítallo Emídio Lira</creatorcontrib><creatorcontrib>Niemeyer, Samira Helena</creatorcontrib><creatorcontrib>Carvalho, Thiago Saads</creatorcontrib><creatorcontrib>Scaramucci, Taís</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakae, Letícia Oba</au><au>Renzo, Ana Luísa Meira</au><au>Viana, Ítallo Emídio Lira</au><au>Niemeyer, Samira Helena</au><au>Carvalho, Thiago Saads</au><au>Scaramucci, Taís</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of different brushing/abrasion protocols on erosive tooth wear for in vitro studies</atitle><jtitle>Archives of oral biology</jtitle><addtitle>Arch Oral Biol</addtitle><date>2023-04</date><risdate>2023</risdate><volume>148</volume><spage>105657</spage><epage>105657</epage><pages>105657-105657</pages><artnum>105657</artnum><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>To investigate the influence of different toothbrushing (with dentifrice) protocols on the progression of erosive tooth wear for in vitro studies. Bovine enamel specimens were randomly distributed into 12 experimental groups (n = 10), according to the study factors: (1) brushing movement (horizontal or circular); (2) slurry diluent (artificial saliva or distilled water); (3) toothpaste dilution ratio (1:2, 1:3 or 1:4). A 5-day erosion-abrasion cycling model was performed, each consisting of 4 erosive challenges (0.3 % citric acid, pH=2.6) followed by 60 min exposure to artificial saliva. Brushing with fluoride toothpaste (15 s, 1400 ppm F-, AmF) was carried out 2x/day. Enamel surface loss (SL) was determined by optical profilometry. Data were statistically analyzed with three-way ANOVA and Tukey tests (α = 0.05). SL was lower for the horizontal movement than for the circular (p = 0.044). There were no significant differences among the dilution ratios for artificial saliva. For distilled water, the more concentrated slurry (1:2) presented greater surface loss than the less concentrated slurries (1:3 and 1:4, p = 0.049 and p = 0.014, respectively). Dilutions with artificial saliva at ratios 1:3 and 1:4 presented higher surface loss than with distilled water (p = 0.008 and p &lt; 0.001, respectively); however, for 1:2 ratio, there were no significant differences between the diluents. The in vitro progression of enamel SL was influenced by the brushing movement, as well as the combination of the dilution ratio and the diluent of the toothpaste slurry, and therefore, all these factors must be considered when comparing results from different studies. •Designing the brushing protocol of ETW studies is complex and challenging.•ETW is influenced by the brushing movement, slurry diluent and dilution ratio.•Standardization of ETW protocols is desirable to allow comparison between studies.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>36827929</pmid><doi>10.1016/j.archoralbio.2023.105657</doi><tpages>1</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0003-9969
ispartof Archives of oral biology, 2023-04, Vol.148, p.105657-105657, Article 105657
issn 0003-9969
1879-1506
language eng
recordid cdi_proquest_miscellaneous_2780075936
source ScienceDirect Freedom Collection
subjects Animals
Cattle
Enamel
Erosive tooth wear
Saliva, Artificial
Study design
Surface loss
Tooth Abrasion
Tooth Erosion
Tooth Wear
Toothbrushing
Toothpastes
Water
title Impact of different brushing/abrasion protocols on erosive tooth wear for in vitro studies
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T23%3A35%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Impact%20of%20different%20brushing/abrasion%20protocols%20on%20erosive%20tooth%20wear%20for%20in%20vitro%20studies&rft.jtitle=Archives%20of%20oral%20biology&rft.au=Sakae,%20Let%C3%ADcia%20Oba&rft.date=2023-04&rft.volume=148&rft.spage=105657&rft.epage=105657&rft.pages=105657-105657&rft.artnum=105657&rft.issn=0003-9969&rft.eissn=1879-1506&rft_id=info:doi/10.1016/j.archoralbio.2023.105657&rft_dat=%3Cproquest_cross%3E2780075936%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c377t-85a8521a1753845d7860ec13c0cfd6b81f725852e16ab911eefa24911f9f303e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2780075936&rft_id=info:pmid/36827929&rfr_iscdi=true