Shrimp Glucose-6-phosphatase 2 (G6Pase 2): a second isoform of G6Pase in the Pacific white shrimp and regulation of G6Pase 1 and 2 isoforms via HIF-1 during hypoxia and reoxygenation in juveniles

• Published in: Journal of bioenergetics and biomembranes 2023-04, Vol.55 (2), p.137-150
• Main Authors: Hernández-Aguirre, Laura E., Peregrino-Uriarte, Alma B., Duarte-Gutiérrez, Jorge L., Leyva-Carrillo, Lilia, Ezquerra-Brauer, Josafat M., Valenzuela-Soto, Elisa M., Yepiz-Plascencia, Gloria
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Biochemistry; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Dissolved oxygen; Gene expression; Gene silencing; Gills; Gluconeogenesis; Glucose; Glucose-6-phosphatase; Glucose-6-Phosphatase - genetics; Glucose-6-Phosphatase - metabolism; Hepatopancreas; Histology; Homeostasis; Hypoxia; Hypoxia - metabolism; Hypoxia-inducible factor 1; Isoforms; Morphology; Muscles; Organic Chemistry; Oxygen; Oxygen - metabolism; Oxygen consumption; Penaeidae - genetics; Penaeidae - metabolism; Phosphatase; Protein Isoforms - metabolism; Shrimps
• ISSN: 0145-479X; 1573-6881
• DOI: 10.1007/s10863-023-09960-z

Animals suffer hypoxia when their oxygen consumption is larger than the oxygen available. Hypoxia affects the white shrimp Penaeus (Litopenaeus) vannamei , both in their natural habitat and in cultivation farms. Shrimp regulates some enzymes that participate in energy production pathways as a strategy to survive during hypoxia. Glucose-6-phosphatase (G6Pase) is key to maintain blood glucose homeostasis through gluconeogenesis and glycogenolysis. We previously reported a shrimp G6Pase gene (G6Pase1) and in this work, we report a second isoform that we named G6Pase2. The expression of the two isoforms was evaluated in oxygen limited conditions and during silencing of the transcription factor HIF-1. High G6Pase activity was detected in hepatopancreas followed by muscle and gills under good oxygen and feeding conditions. Gene expression of both isoforms was analyzed in normoxia, hypoxia and reoxygenation in hepatopancreas and gills, and in HIF-1-silenced shrimp. In fed shrimp with normal dissolved oxygen (DO) (5.0 mg L − 1 DO) the expression of G6Pase1 was detected in gills, but not in hepatopancreas or muscle, while G6Pase2 expression was undetectable in all three tissues. In hepatopancreas, G6Pase1 is induced at 3 and 48 h of hypoxia, while G6Pase2 is down-regulated in the same time points but in reoxygenation, both due to the knock-down of HIF-1. In gills, only G6Pase1 was detected, and was induced by the silencing of HIF-1 only after 3 h of reoxygenation. Therefore, the expression of the two isoforms appears to be regulated by HIF-1 at transcriptional level in response to oxygen deprivation and subsequent recovery of oxygen levels.