Isolation of metallic salts of cytotoxic clerodanes from medicinal plant Polyalthia longifolia var. pendula

• Published in: Pakistan journal of pharmaceutical sciences 2022-11, Vol.35 (6(Special)), p.1691-1698
• Main Authors: Shamshad, Shumaila, Versiani, Muhammad A, Ikram, Ambreen, Yasmeen, Kousar, A Khan, Rashid, R Mughal, Najma, Ahmed, Ayaz, Muzafar, Wajeeha, Faizi, Shaheen
• Format: Article
• Language: English
• Subjects: Animals; Annonaceae; Antineoplastic Agents - pharmacology; Diterpenes, Clerodane - pharmacology; Mice; Plants, Medicinal; Polyalthia; Potassium; Salts
• ISSN: 1011-601X
• DOI: 10.36721/PJPS.2022.35.6.SP.1691-1698.1

Isolation of sodium and potassium salt of kolavenic acid (1,2), as a mixture of (3:1) and sodium and potassium salt of 16 oxo-cleroda-3,13(14) E-dien-15-oic acid (3, 4) as a mixture of (1:1) are first time reported form reddish black ripe and green unripe berries of Polyalthia longifolia var. pendula respectively. Three known constituents obtained, were identified as cleroda-3, 13(14) E-dien-15-oic acid (kolavenic acid) (5), 16(R and S)-hydroxy cleroda-3,13 (14)Z-dien-15,16-olide (6) and 16 oxo-cleroda-3,13(14) E-dien-15-oic acid (7). Structures of all these compounds have been determined through spectral studies while metal analyses were carried out to confirm the structure of the salts. Compounds 3, 4 and 7 possess cytotoxic activity against lung (NCI-H460), oral (CAL-27) and normal mouse fibroblast (NCI-3T3) cancer cell lines. Diterpenoid (7), a bioprivileged, compound shows potent cytotoxic activity against oral cancer cell line (CAL-27) with IC 11.3±0.6µg/mL in comparison with the standard 5-flourouracil (IC 12.7±0.1µg/mL) and lungs cancer cell lines (NCI-H460) with IC 5.3±0.2µg/mL as compared to the standard drug cisplatin (IC 5.7±0.2µg/mL).