Characterization of polypropyleneimine as an alternative transfection reagent

Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than...

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Bibliographic Details
Published in:Analytical sciences 2023-06, Vol.39 (6), p.1015-1020
Main Authors: Saeki, Riku, Kobayashi, Shingo, Shimazui, Rena, Nii, Teruki, Kishimura, Akihiro, Mori, Takeshi, Tanaka, Masaru, Katayama, Yoshiki
Format: Article
Language:English
Subjects:
Analytical Chemistry
Cell Line
Chemistry
Chemistry and Materials Science
Indicators and Reagents
Plasmids - genetics
Polyethyleneimine - chemistry
Polypropylenes
Transfection
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Summary:Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent. Graphical abstract
ISSN:0910-6340
1348-2246
DOI:10.1007/s44211-023-00284-x