Variability in growth and biofilm formation of Listeria monocytogenes in Agaricus bisporus mushroom products

• Published in: Food research international 2023-03, Vol.165, p.112488-112488, Article 112488
• Main Authors: Lake, Frank B., van Overbeek, Leo S., Baars, Johan J.P., Abee, Tjakko, den Besten, Heidy M.W.
• Format: Article
• Language: English
• Subjects: Agaricus; Biofilm formation; Biofilms; Growth performance; Humans; Listeria monocytogenes; Mannitol; Mushroom medium; Natural microbiota; Nutrient consumption
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2023.112488

[Display omitted] •Filter-sterilized mushroom medium is a suitable nutrient source for L. monocytogenes.•Variability in growth performance between L. monocytogenes mushroom isolates is low.•L. monocytogenes mushroom isolates and non-mushroom isolates have comparable growth.•Mushroom products support grow of L. monocytogenes despite high background microbiota. Foods and food production environments can be contaminated with Listeria monocytogenes and may support growth of this foodborne pathogen. This study aims to characterize the growth and biofilm formation of sixteen L. monocytogenes strains, isolated from mushroom production and processing environments, in filter-sterilized mushroom medium. Strain performance was compared to twelve L. monocytogenes strains isolated from other sources including food and human isolates. All twenty-eight L. monocytogenes strains showed rather similar growth performance at 20 °C in mushroom medium, and also significant biofilm formation was observed for all strains. HPLC analysis revealed the presence of mannitol, trehalose, glucose, fructose and glycerol, that were all metabolized by L. monocytogenes, except mannitol, in line with the inability of L. monocytogenes to metabolize this carbohydrate. Additionally, the growing behavior of L. monocytogenes was tested on whole, sliced and smashed mushroom products to quantify performance in the presence of product-associated microbiota. A significant increase of L. monocytogenes was observed with higher increase of counts when the mushroom products were more damaged, even with the presence of high background microbiota counts. This study demonstrated that L. monocytogenes grows well in mushroom products, even when the background microbiota is high, highlighting the importance to control (re)contamination of mushrooms.