Chemoreceptors from the commensal gut Roseburia rectibacter bind to mucin and trigger chemotaxis

Chemotaxis is crucial for bacterial adherence and colonization of the host gastrointestinal tract. Previous studies have demonstrated that chemotaxis affects the virulence of causative pathogens and the infection in the host. However, the chemotactic abilities of non‐pathogenic and commensal gut bac...

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Bibliographic Details
Published in:Environmental microbiology 2023-07, Vol.25 (7), p.1329-1343
Main Authors: Chen, Hong‐He, Wang, Yu‐Xin, Li, De‐Feng, Liu, Chang, Bi, Shuang‐Yu, Jiang, Cheng‐Ying, Liu, Shuang‐Jiang
Format: Article
Language:English
Subjects:
Bacteria
Bacteria - metabolism
Bacterial Proteins - metabolism
Chemoreceptors
Chemotaxis
Colonization
Comamonas testosteroni
Digestive system
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Flagella
Gastrointestinal system
Gastrointestinal tract
Genes
Genomes
Ligands
Microorganisms
Mucin
Mucins - metabolism
Pathogens
Propionates - metabolism
Propionic acid
Roseburia rectibacter
Virulence
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Summary:Chemotaxis is crucial for bacterial adherence and colonization of the host gastrointestinal tract. Previous studies have demonstrated that chemotaxis affects the virulence of causative pathogens and the infection in the host. However, the chemotactic abilities of non‐pathogenic and commensal gut bacteria have rarely been explored. We observed that Roseburia rectibacter NSJ‐69 exhibited flagella‐dependent motility and chemotaxis to a variety of molecules, including mucin and propionate. A genome‐wide analysis revealed that NSJ‐69 has 28 putative chemoreceptors, 15 of which have periplasmic ligand‐binding domains (LBDs). These LBD‐coding genes were chemically synthesized and expressed heterologously in Escherichia coli. Intensive screening of ligands revealed four chemoreceptors bound to mucin and two bound to propionate. When expressed in Comamonas testosteroni or E. coli, these chemoreceptors elicited chemotaxis toward mucin and propionate. Hybrid chemoreceptors were constructed, and results showed that the chemotactic responses to mucin and propionate were dependent on the LBDs of R. rectibacter chemoreceptors. Our study identified and characterized R. rectibacter chemoreceptors. These results will facilitate further investigations on the involvement of microbial chemotaxis in host colonization.
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.16365