Cytokine enrichment in equine conditioned serum is not reliant on incubation in specialized containers

Autologous conditioned serum (ACS), i.e serum enriched with anti-inflammatory cytokines and growth factors, is a popular orthobiologic therapy used in equine practice. Costly specialized tubes containing glass beads are commonly used for ACS production. The objective of this in vitro study was to co...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2023-04, Vol.258, p.110576-110576, Article 110576
Main Authors: Nakken, Gina, Kirk, Joseph, Fjordbakk, Cathrine T.
Format: Article
Language:English
Subjects:
Animals
Autologous conditioned serum
Becaplermin
Cytokines - metabolism
Equine
Horses
Insulin-Like Growth Factor I - metabolism
Interleukin 1 Receptor Antagonist Protein
Orthobiologics
Serum - metabolism
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Summary:Autologous conditioned serum (ACS), i.e serum enriched with anti-inflammatory cytokines and growth factors, is a popular orthobiologic therapy used in equine practice. Costly specialized tubes containing glass beads are commonly used for ACS production. The objective of this in vitro study was to compare cytokine and growth factor levels in equine serum after incubation in three different tubes: commercial plastic ACS tubes (COMM); sterile 50 ml plastic centrifugation tubes (CEN); and 10 ml plastic vacutainer tubes (VAC). Blood from 15 healthy horses was incubated in the different tubes at 37°C for 22–24 h. The concentration of IL-1β, IL-1Ra, IL-10, IGF-1 and PDGF-BB was determined by ELISA and compared between tubes. There was no difference in concentration of IL-1Ra and IGF-1 between CEN and COMM. PDGF-BB was higher in CEN vs. COMM (P 
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2023.110576