Influence of temperature on the biomarker responses of bullfrog tadpoles (Lithobates catesbeianus) to 2-hydroxyatrazine exposure

•2-hydroxyatrazine (2-HA) exposure decreased SOD and increased GST activities in tadpoles.•2-HA caused histopathological alterations in liver, but especially in kidneys of tadpoles.•2-HA was non-genotoxic to tadpoles at tested conditions.•Temperature influenced biomarker responses of tadpoles to 2-H...

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Published in:Aquatic toxicology 2023-04, Vol.257, p.106468-106468, Article 106468
Main Authors: Carneiro, Francisco Estevão, Grott, Suelen Cristina, Israel, Nicole Grasmuk, Bitschinski, Daiane, Abel, Gustavo, Alves, Thiago Caique, de Albuquerque, Claudia Almeida Coelho, da Silva, Elizia Barbosa, de Almeida, Eduardo Alves
Format: Article
Language:English
Subjects:
2-hydroxyatrazine
Acetylcholinesterase - metabolism
Animals
Atrazine - metabolism
Biomarker
Biomarkers - metabolism
Genotoxicity
Histopathology
Larva - metabolism
Lithobates catesbeianus
Oxidative stress
Rana catesbeiana
Temperature
Water Pollutants, Chemical - toxicity
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Summary:•2-hydroxyatrazine (2-HA) exposure decreased SOD and increased GST activities in tadpoles.•2-HA caused histopathological alterations in liver, but especially in kidneys of tadpoles.•2-HA was non-genotoxic to tadpoles at tested conditions.•Temperature influenced biomarker responses of tadpoles to 2-HA. The influence of temperature (25 and 32 °C) on the biomarker responses of bullfrog tadpoles (Lithobates catesbeianus) to different concentrations of the atrazine metabolite 2-hydroxyatrazine (2-HA, 0, 10, 50 and 200 ng.L−1, 16 days), was evaluated. Temperature affected the activities of superoxide dismutase, glutathione S-transferase and acetylcholinesterase. The activities of catalase, glutathione peroxidase, glucose-6-phosphate dehydrogenase and carboxylesterase presented no alterations. Frequencies of micronuclei and nuclear abnormalities were also not altered. 2-HA decreased SOD activity at 25 °C and caused histopathological changes in the liver and the kidney at both temperatures, with the kidney being more affected by the combination of higher temperature and 2-HA exposure, presenting glomerular shrinkage and an increase in Bowman's space. Our results indicate that at environmentally relevant concentrations, 2-HA can cause changes in biomarker responses as well as in the morphology of liver and kidney in L. catesbeianus tadpoles. Temperature has an important influence on biomarker response and histopathological alterations.
ISSN:0166-445X
1879-1514
DOI:10.1016/j.aquatox.2023.106468