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Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy
[Display omitted] Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethas...
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Published in: | International journal of pharmaceutics 2023-04, Vol.636, p.122808-122808, Article 122808 |
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container_title | International journal of pharmaceutics |
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creator | Pena-Rodríguez, Eloy García-Berrocoso, Teresa Vázquez Fernández, Ezequiel Otero-Espinar, Francisco J. Abian, Joaquin Fernández-Campos, Francisco |
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Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 μm) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions. |
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Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 μm) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2023.122808</identifier><identifier>PMID: 36889415</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Biodistribution ; Confocal Raman ; Dexamethasone ; MALDI-MSI ; Microscopy ; Nanoparticle ; Semiquantitative ; Skin ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Tissue Distribution</subject><ispartof>International journal of pharmaceutics, 2023-04, Vol.636, p.122808-122808, Article 122808</ispartof><rights>2023 The Author(s)</rights><rights>Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-d7f5aedff4d4289876011d62dc871631b0590aade5388218fc6ffd73b3ca19383</citedby><cites>FETCH-LOGICAL-c412t-d7f5aedff4d4289876011d62dc871631b0590aade5388218fc6ffd73b3ca19383</cites><orcidid>0000-0001-8224-2932 ; 0000-0001-8072-8533 ; 0000-0001-8743-7229</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36889415$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pena-Rodríguez, Eloy</creatorcontrib><creatorcontrib>García-Berrocoso, Teresa</creatorcontrib><creatorcontrib>Vázquez Fernández, Ezequiel</creatorcontrib><creatorcontrib>Otero-Espinar, Francisco J.</creatorcontrib><creatorcontrib>Abian, Joaquin</creatorcontrib><creatorcontrib>Fernández-Campos, Francisco</creatorcontrib><title>Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>[Display omitted]
Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 μm) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions.</description><subject>Biodistribution</subject><subject>Confocal Raman</subject><subject>Dexamethasone</subject><subject>MALDI-MSI</subject><subject>Microscopy</subject><subject>Nanoparticle</subject><subject>Semiquantitative</subject><subject>Skin</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Tissue Distribution</subject><issn>0378-5173</issn><issn>1873-3476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFkMtuEzEUhi0EoqHlEUBespngy4ztWaGqtFApVSXUri2PL80JGXuwJ6GRePjOKIEtq7P5L-f_EPpAyZISKj5vlrAZ1ib3S0YYX1LGFFGv0IIqySteS_EaLQiXqmqo5GfoXSkbQohglL9FZ1wo1da0WaA_dynCmDLEJ-z8s-n9uDYlRY_LT4i4g-SgjBm63Qgp4t8wrrF_xnvYJ3x3ufp6Wz3c3-DelILL4O2Y05SQDxh68zRnmuiwTTEka7b4h-lNxD3YnIpNw-ECvQlmW_z70z1HjzfXD1ffq9X9t9ury1Vla8rGysnQGO9CqF3NVKukIJQ6wZxVkgpOO9K0xBjnG64UoypYEYKTvOPW0JYrfo4-HXOHnH7tfBl1D8X67dZEn3ZFM6ka2oqWzNLmKJ1_LNkHPeRpSz5oSvQMXm_0Cbyewesj-Mn38VSx63rv_rn-kp4EX44CPw3dg8-6WPDRegd54qZdgv9UvAC-VJk8</recordid><startdate>20230405</startdate><enddate>20230405</enddate><creator>Pena-Rodríguez, Eloy</creator><creator>García-Berrocoso, Teresa</creator><creator>Vázquez Fernández, Ezequiel</creator><creator>Otero-Espinar, Francisco J.</creator><creator>Abian, Joaquin</creator><creator>Fernández-Campos, Francisco</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8224-2932</orcidid><orcidid>https://orcid.org/0000-0001-8072-8533</orcidid><orcidid>https://orcid.org/0000-0001-8743-7229</orcidid></search><sort><creationdate>20230405</creationdate><title>Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy</title><author>Pena-Rodríguez, Eloy ; García-Berrocoso, Teresa ; Vázquez Fernández, Ezequiel ; Otero-Espinar, Francisco J. ; Abian, Joaquin ; Fernández-Campos, Francisco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-d7f5aedff4d4289876011d62dc871631b0590aade5388218fc6ffd73b3ca19383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biodistribution</topic><topic>Confocal Raman</topic><topic>Dexamethasone</topic><topic>MALDI-MSI</topic><topic>Microscopy</topic><topic>Nanoparticle</topic><topic>Semiquantitative</topic><topic>Skin</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pena-Rodríguez, Eloy</creatorcontrib><creatorcontrib>García-Berrocoso, Teresa</creatorcontrib><creatorcontrib>Vázquez Fernández, Ezequiel</creatorcontrib><creatorcontrib>Otero-Espinar, Francisco J.</creatorcontrib><creatorcontrib>Abian, Joaquin</creatorcontrib><creatorcontrib>Fernández-Campos, Francisco</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pena-Rodríguez, Eloy</au><au>García-Berrocoso, Teresa</au><au>Vázquez Fernández, Ezequiel</au><au>Otero-Espinar, Francisco J.</au><au>Abian, Joaquin</au><au>Fernández-Campos, Francisco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2023-04-05</date><risdate>2023</risdate><volume>636</volume><spage>122808</spage><epage>122808</epage><pages>122808-122808</pages><artnum>122808</artnum><issn>0378-5173</issn><eissn>1873-3476</eissn><abstract>[Display omitted]
Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 μm) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36889415</pmid><doi>10.1016/j.ijpharm.2023.122808</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-8224-2932</orcidid><orcidid>https://orcid.org/0000-0001-8072-8533</orcidid><orcidid>https://orcid.org/0000-0001-8743-7229</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Biodistribution Confocal Raman Dexamethasone MALDI-MSI Microscopy Nanoparticle Semiquantitative Skin Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Tissue Distribution |
title | Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy |
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