Bone marrow microenvironment disruption and sustained inflammation with prolonged haematologic toxicity after CAR T‐cell therapy

Summary Mechanisms of prolonged cytopenia (PC) after chimeric antigen receptor (CAR) T‐cell therapy, an emerging therapy for relapsed or refractory diffuse large B‐cell lymphoma, remain elusive. Haematopoiesis is tightly regulated by the bone marrow (BM) microenvironment, called the ‘niche’. To inve...

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Bibliographic Details
Published in:British journal of haematology 2023-07, Vol.202 (2), p.294-307
Main Authors: Kitamura, Wataru, Asada, Noboru, Naoi, Yusuke, Abe, Masaya, Fujiwara, Hideaki, Ennishi, Daisuke, Nishimori, Hisakazu, Fujii, Keiko, Fujii, Nobuharu, Matsuoka, Ken‐ichi, Yoshino, Tadashi, Maeda, Yoshinobu
Format: Article
Language:English
Subjects:
Antigens, CD19
Biopsy
Bone Marrow
bone marrow niche
Cell therapy
chimeric antigen receptor T cell
Chimeric antigen receptors
CXC chemokines
Cytokines
Cytopenia
diffuse large B‐cell lymphoma
Hematology
Hemopoiesis
Humans
Immunotherapy, Adoptive - adverse effects
Immunotherapy, Adoptive - methods
Inflammation
Leukopenia
Lymphoma
Lymphoma, Large B-Cell, Diffuse - therapy
Lymphoma, Non-Hodgkin
Microenvironments
prolonged cytopenia
Stem cell factor
Stromal cells
Toxicity
Tumor Microenvironment
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Summary:Summary Mechanisms of prolonged cytopenia (PC) after chimeric antigen receptor (CAR) T‐cell therapy, an emerging therapy for relapsed or refractory diffuse large B‐cell lymphoma, remain elusive. Haematopoiesis is tightly regulated by the bone marrow (BM) microenvironment, called the ‘niche’. To investigate whether alterations in the BM niche cells are associated with PC, we analysed CD271+ stromal cells in BM biopsy specimens and the cytokine profiles of the BM and serum obtained before and on day 28 after CAR T‐cell infusion. Imaging analyses of the BM biopsy specimens revealed that CD271+ niche cells were severely impaired after CAR T‐cell infusion in patients with PC. Cytokine analyses after CAR T‐cell infusion showed that CXC chemokine ligand 12 and stem cell factor, niche factors essential for haematopoietic recovery, were significantly decreased in the BM of patients with PC, suggesting reduced niche cell function. The levels of inflammation‐related cytokines on day 28 after CAR T‐cell infusion were consistently high in the BM of patients with PC. Thus, we demonstrate for the first time that BM niche disruption and sustained elevation of inflammation‐related cytokines in the BM following CAR T‐cell infusion are associated with subsequent PC.
ISSN:0007-1048
1365-2141
DOI:10.1111/bjh.18747