Vancomycin Loaded Amino-Functionalized MCM-48 Mesoporous Silica Nanoparticles as a Promising Drug Carrier in Bone Substitutes for Bacterial Infection Management

• Published in: Applied biochemistry and biotechnology 2023-11, Vol.195 (11), p.6607-6632
• Main Authors: Rahaman, Syed Nasar, Pathmanapan, Srinivetha, Sidharthan, Anbarasi, Anandasadagopan, Suresh Kumar
• Format: Article
• Language: English
• Subjects: Antibacterial activity; Antiinfectives and antibacterials; Bacteria; Bacterial diseases; Bacterial infections; Biochemistry; Biocompatibility; Biofilms; Biomaterials; Biomedical materials; Biotechnology; Bone biomaterials; Bone cements; Bone implants; Cement; Chemistry; Chemistry and Materials Science; Controlled release; Cytotoxicity; Disintegration; Drug carriers; Drug delivery; Electron microscopy; Emission analysis; Field emission microscopy; Fourier transforms; Infrared spectroscopy; Light scattering; Mechanical loading; Membranes; Microscopy; Nanoparticles; Original Article; Orthopedics; Photon correlation spectroscopy; Scanning electron microscopy; Silica; Silicon dioxide; Staphylococcus aureus; Substitute bone; Surgical implants; Tissue engineering; Transmission electron microscopy; Vancomycin; Zeta potential
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-023-04406-z

Orthopedic infections due to biofilm formation in biomaterial-based implants have become challenging in bone tissue engineering. In the present study, in vitro antibacterial analysis of amino-functionalized MCM-48 mesoporous silica nanoparticles (AF-MSNs) loaded with vancomycin is analyzed for its potential as a drug carrier for the sustained/controlled release of vancomycin against Staphylococcus aureus . The effective incorporation of vancomycin into the inner core of AF-MSNs was observed by alternation in the absorption frequencies obtained by Fourier transform infrared spectroscopy (FTIR). Dynamic light scattering (DLS) and high resolution-transmission electron microscopy (HR-TEM) results show that all the AF-MSNs had homogeneous spherical shapes with a mean diameter of 165.2 ± 1.25 nm, and there is a slight change in the hydrodynamic diameter after vancomycin loading. Furthermore, the zeta potential of all the AF-MSNs (+ 30.5 ± 0.54 mV) and AF-MSN/VA (+ 33.3 ± 0.56 mV) were positively charged due to effective functionalization with 3-aminopropyl triethoxysilane (APTES). Furthermore, cytotoxicity results show that the AF-MSNs have better biocompatibility than non-functionalized MSNs ( p