Study on extraction and characterization of new antibiotics violacein from engineered Escherichia coli VioABCDE-SD

To better understand the characteristic properties of violacein biosynthesized by engineered Escherichia coli VioABCDE-SD, a convenient and simplified method was designed to extract violacein and its stability, antimicrobial activity, and antioxidant capacity were analyzed. Different from the tradit...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2023-10, Vol.70 (5), p.1582-1596
Main Authors: Huang, Chunyan, Chu, Xiaoting, Hui, Wenyang, Xie, Chengjia, Xu, Xian
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial activity
Antioxidants
Carbon dioxide
Cobalt
Cosmetics
E coli
Escherichia coli
Food additives
Food industry
Free radicals
Hydroxyl radicals
Low temperature
Reagents
Reducing agents
Scavenging
Stability analysis
Sucrose
Violacein
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Summary:To better understand the characteristic properties of violacein biosynthesized by engineered Escherichia coli VioABCDE-SD, a convenient and simplified method was designed to extract violacein and its stability, antimicrobial activity, and antioxidant capacity were analyzed. Different from the traditional extraction methods, our new method is easier and less time consuming and can directly obtain violacein dry powder product with a higher extraction rate. Low temperature, dark condition, neutral pH, reducing agents, Ba , Mn , Ni , Co , and some food additives of sucrose, xylose, and glucose were conducive to maintaining its stability. The violacein also exhibited surprisingly high bacteriostatic action against Gram-positive Bacillus subtilis, Deinococcus radiodurans R1, and Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa, but no effect on E. coli. The violacein of VioABCDE-SD exhibited strong antioxidant activity, with the scavenging rate of 1,1-diphenyl-2-picrylhydrazyl free radicals reaching 60.33%, the scavenging efficiency of hydroxyl radical scavenging reaching 56.34%, and the total antioxidant capacity reaching 0.63 U/mL. Violacein from VioABCDE-SD can be synthesized directionally with better stability, antibacterial, and antioxidant properties compared with that from the original strain Janthinobacterium sp. B9-8. Therefore, our study indicated that violacein from engineered E. coli VioABCDE-SD was a kind of new antibiotic with potential biological activities, which may have potential utility in multiple areas such as pharmacological, cosmetics, and healthy food industries.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.2454