Structure of the lipopolysaccharide O-antigen of endophytic Pseudomonas sp. strain L1

• Published in: Carbohydrate research 2023-03, Vol.525, p.108779-108779, Article 108779
• Main Authors: Choma, Adam, Kaczyński, Zbigniew, Komaniecka, Iwona, Swatek, Anita, Kasperkiewicz, Katarzyna, Pawlik, Małgorzata, Piotrowska-Seget, Zofia
• Format: Article
• Language: English
• Subjects: Carbohydrate Sequence; Endophytes; Lipopolysaccharide; Lipopolysaccharides - chemistry; Magnetic Resonance Spectroscopy; MALDI-TOF; NMR; O Antigens - chemistry; O-specific polysaccharide; Pseudomonas; Pseudomonas sp
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2023.108779

The O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide of Pseudomonas sp. Strain L1, the endophytic bacteria of Lolium perenne (ryegrass) plants growing in soil in an industrial area in the Silesia region (Zabrze, Southern Poland). The high-molecular-weight O-PS fraction liberated from Pseudomonas sp. L1 lipopolysaccharide by mild acid hydrolysis was studied using chemical methods, MALDI-TOF mass spectrometry, and 1D and 2D NMR spectroscopy techniques. It was found that the O-specific polysaccharide was built of tetrasaccharide repeating units composed of d-FucpN, d-Fucp4N, and two d-QuipN residues. The following structure of the O-PS of Pseudomonas sp. Strain L1 was established:→4)-a-d-FucpN-(1→3)-a-d-QuipN-(1→3)-β-d-QuipN-(1→3)-a-d-Fucp4N-(1→ [Display omitted] •The O-polysaccharide was isolated from endophytic Pseudomonas sp. L1.•The chemical structure of the O-PS was determined using chemical methods and NMR spectroscopy.•The O-PS consists exclusively of 6-deoxyamino sugars (i.e. FucN, QuiN, and Fuc4N).•The O-PS repeating unit contains four monosugar residues.