Seed-mediated strategy for synthesis of enzyme-encapsulated metal-organic frameworks with enhanced enzyme activity

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2023-05, Vol.225, p.113246-113246, Article 113246
• Main Authors: Huang, Zhiyang, Liang, Jianwei, Wang, Kuiyu, Yang, Tao, Zeng, Hui
• Format: Article
• Language: English
• Subjects: Bioactivity; Biomaterials; Enzyme encapsulation; Enzymes, Immobilized - metabolism; Metal-Organic Frameworks; Non-modified; Nucleation; Proteins; Seed
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2023.113246

Encapsulation of enzymes into metal-organic frameworks (enzyme@MOF) can improve the stability of enzymes. Most present synthesis methods of enzyme@MOF rely on the complex modification of enzymes or the natural negative surface charge of enzymes to promote the synthesis of enzyme@MOF. Despite extensive efforts, it remains challenging to develop a surface charge-independent and convenient strategy to encapsulate various enzymes into MOF efficiently. In this study, we proposed a convenient seed-mediated strategy for efficient synthesis of enzyme@MOF from the perspective of MOF formation. The seed, acting as nuclei, makes the slow nucleation stage skipped, leading to the efficient synthesis of enzyme@MOF. The successful encapsulation of several proteins demonstrated the feasibility and advantages of the seed-mediated strategy. Moreover, the resulting composite, cytochrome (Cyt c) encapsulated in ZIF-8, exhibited a 5.6-fold increase in bioactivity compared to free Cyt c. The seed-mediated strategy provides an efficient, enzyme surface charge-independent, and non-modified method for the synthesis of enzyme@MOF biomaterials, which warrants further exploration and application in diverse fields. [Display omitted] •Seed enables synthesis of enzyme-encapsulated metal-organic framework(enzyme@MOF).•The seed which acts as the nucleus greatly promotes the formation of enzyme@MOF.•A one-pot synthesis method for enzyme@MOF avoids complex modification of enzymes.•The activity of encapsulated Cytochrome c was 6.6 times that of free Cytochrome c.