CRISPR/Cas9 Screen in Gastric Cancer Patient‐Derived Organoids Reveals KDM1A‐NDRG1 Axis as a Targetable Vulnerability

• Published in: Small methods 2023-06, Vol.7 (6), p.e2201605-n/a
• Main Authors: Mircetic, Jovan, Camgöz, Aylin, Abohawya, Moustafa, Ding, Li, Dietzel, Julia, Tobar, Sebastián García, Paszkowski‐Rogacz, Maciej, Seidlitz, Therese, Schmäche, Tim, Mehnert, Marie‐Christin, Sidorova, Olga, Weitz, Jürgen, Buchholz, Frank, Stange, Daniel E.
• Format: Article
• Language: English
• Subjects: Cell Line, Tumor; CRISPR screen; CRISPR-Cas Systems - genetics; epigenetic regulators; gastric cancer; Histone Demethylases - genetics; Histone Demethylases - metabolism; Humans; KDM1A‐NDRG1 axis; Organoids - metabolism; Organoids - pathology; patient‐derived organoids; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology
• ISSN: 2366-9608; 2366-9608
• DOI: 10.1002/smtd.202201605

Viability CRISPR screens have proven indispensable in parsing genome function. However, their application in new, more physiologically relevant culturing systems like patient‐derived organoids (PDOs) has been much slower. To probe epigenetic contribution to gastric cancer (GC), the third leading cause of cancer‐related deaths worldwide, the first negative selection CRISPR screen in GC PDOs that faithfully preserve primary tumor characteristics is performed. Extensive quality control measurements showing feasibility of CRISPR screens in primary organoid culture are provided. The screen reveals the histone lysine demethylase‐1A (KDM1A) to constitute a GC vulnerability. Both genetic and pharmacological inhibition of KDM1A cause organoid growth retardation. Further, it is shown that most of KDM1A cancer‐supporting functions center on repression of N‐myc downstream regulates gene‐1 (NDRG1). De‐repression of NDRG1 by KDM1A inhibitors (KDM1Ai) causes inhibition of Wnt signaling and a strong G1 cell cycle arrest. Finally, by profiling 20 GC PDOs, it is shown that NDRG1 upregulation predicts KDM1Ai response with 100% sensitivity and 82% specificity in the tested cohort. Thus, this work pioneers the use of negative selection CRISPR screens in patient‐derived organoids, identifies a marker of KDM1Ai response, and accordingly a cohort of patients who may benefit from such therapy. Patient‐derived organoids constitute an invaluable system to model disease, including cancer. By carefully assessing and adjusting screening parameters, a high‐quality CIRSPR screen in gastric cancer organoids is performed. The negative selection screen identifies multiple genes important for cancer cell viability. Focusing on the histone demethylase KDM1A, a response marker of a novel potential treatment option is uncovered.