Gastric epithelial response to milk fat using the semi-dynamic INFOGEST digestion model coupled with NCI-N87 cells

[Display omitted] •Gastric epithelial molecular response to fat-free and whole milks was studied.•A semi-dynamic in vitro digestion method combined with NCI-N87 cells was used.•The selected milk samples up-regulated CAT mRNA expression equally.•Whole milks induced higher NFκB p65 and IL-1β mRNA expr...

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Published in:Food research international 2023-04, Vol.166, p.112576-112576, Article 112576
Main Authors: Pinho, Susana C.M., Faria, Miguel A., Alves, Rui, Cabrita, Ana R.J., Fonseca, António J.M., M.P.L.V.O. Ferreira, Isabel
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Conventional milk
Digestion
Fatty Acids
Gastric models
Gastric Mucosa - metabolism
Humans
Inflammation - metabolism
Interferon-γ
Interleukin-6
Interleukin-8 - genetics
Milk - metabolism
NF-kappa B
Pasture-based milk
Quantitative PCR
RNA, Messenger - genetics
Superoxide Dismutase
Tumor Necrosis Factor-alpha - genetics
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Summary:[Display omitted] •Gastric epithelial molecular response to fat-free and whole milks was studied.•A semi-dynamic in vitro digestion method combined with NCI-N87 cells was used.•The selected milk samples up-regulated CAT mRNA expression equally.•Whole milks induced higher NFκB p65 and IL-1β mRNA expression than fat-free milk.•After IFN-γ-stimulation, whole milks had no effect in NFκB p65 and IL-1β expression. The stomach is a relevant spot of lipolysis for milk fat, but research on the effect of digested milk fat in the gastric epithelium is scarce and difficult to evaluate. In the present study, we implemented the semi-dynamic in vitro digestion model of INFOGEST, combined with gastric NCI-N87 cells, to study the effect of fat-free, whole conventional, and whole pasture-based milk on gastric epithelium. Cellular messenger ribonucleic acid (mRNA) expression of membrane fatty acids receptors (GPR41, GPR84), antioxidant enzymes (CAT, SOD, GPX), and inflammatory molecules (NF-κB p65, IL-1β, IL-6, IL-8 and TNF-α) was assessed. No significant differences were observed in mRNA expression of GPR41, GPR84, SOD, GPX, IL-6, IL-8, and TNF-α, after exposure of the NCI-N87 cells to milk digesta samples (p > 0.05). An increase of CAT mRNA expression was observed (p 
ISSN:0963-9969
1873-7145
DOI:10.1016/j.foodres.2023.112576