Ultra-sensitive determination of Ochratoxin A in coffee and tea samples using a novel semi-automated in-syringe based coagulant-assisted fast mycotoxin extraction (FaMEx) technique coupled with UHPLC-MS/MS

[Display omitted] •A novel in-syringe-based coagulant-assisted microextraction technique was developed.•Ferrous sulfate as a coagulation agent exhibited excellent matrix elimination.•This method utilizes semi-automated setups with minimal sample and solvent amounts.•This protocol is a simple, highly...

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Published in:Food chemistry 2023-08, Vol.417, p.135951-135951, Article 135951
Main Authors: Prakasham, Karthikeyan, Gurrani, Swapnil, Shiea, Jentaie, Wu, Ming-Tsang, Wu, Chia-Fang, Lin, Yu-Chia, Tsai, Bongee, Huang, Po-Chin, Andaluri, Gangadhar, Ponnusamy, Vinoth Kumar
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid - methods
Coagulation-Assisted Liquid-Liquid Microextraction
Coffee - chemistry
Coffee and tea samples
Fast Mycotoxin Extraction
LC-MS/MS
Mycotoxins
Mycotoxins - analysis
Ochratoxin A
Syringes
Tandem Mass Spectrometry - methods
Tea
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Summary:[Display omitted] •A novel in-syringe-based coagulant-assisted microextraction technique was developed.•Ferrous sulfate as a coagulation agent exhibited excellent matrix elimination.•This method utilizes semi-automated setups with minimal sample and solvent amounts.•This protocol is a simple, highly-sensitive, low-matrix effect and efficient procedure.•Applied for the analysis of mycotoxin in coffee and tea samples using LC-MS/MS. In this study, we demonstrated a novel semi-automated in-syringe-based coagulant-assisted liquid–liquid microextraction (IS-CGA-LLME) as fast mycotoxin extraction (FaMEx) technique coupled with ultra-high-performance liquid chromatography connected with a tandem-mass spectrometer (UHPLC-MS/MS) for the quantification of mycotoxin (Ochratoxin A, OT-A) in coffee and tea samples. IS-CGA-LLME is a three-step extraction process that includes extraction of OT-A from sample matrix using low-volume solvent extraction, then the extractant was cleaned-up using a coagulation process, and finally, the decolorized/matrix removed sample solution was processed for LLME for target analyte’s pre-concentration. The final extractant was analyzed using UHPLC-MS/MS for OT-A quantification. Under the optimized experimental conditions, highly sensitive detection and quantification limits were obtained at 0.001 and 0.003 ng g−1 for OT-A with excellent extraction recovery (93–111%) and precision
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2023.135951