Screening potential polyhydroxyalkanoate-producing bacteria from wastewater sludge

We applied fluorescence staining of Nile red, polymerase chain reaction (PCR), and carbon substrate utilization and pressure tolerance analysis to execute three-stage screening for potential polyhydroxyalkanoate (PHA) producers in the sludge samples of 21 large-scale wastewater treatment plants of c...

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Bibliographic Details
Published in:Archives of microbiology 2023-04, Vol.205 (4), p.120-120, Article 120
Main Authors: Chou, Hung‑Che, Chen, Chia‑Hsin, Huang, Chun‑Mei, Wang, Hui‑Jun, Hsiung, Yu-Chuan, Liang, Chen-Hsien, Ou, Chung-Mao, Guo, Gia‑Luen
Format: Article
Language:English
Subjects:
Accumulation
Activated sludge
Archives & records
Bacteria
Bacteria - genetics
Biochemistry
Biodiversity
Biomedical and Life Sciences
Bioreactors - microbiology
Biotechnology
Carbon
Carbon sources
Cell Biology
Dry cells
Ecology
Glucose
Industrial applications
Industrial areas
Industrial parks
Industrial plants
Life Sciences
Microbial Ecology
Microbiology
Nitrogen
Original Paper
Polyhydroxyalkanoates
Polyhydroxyalkanoic acid
Polymerase chain reaction
Pressure tolerance
RNA, Ribosomal, 16S - genetics
rRNA 16S
Screening
Sewage - microbiology
Sludge
Substrates
Wastewater
Wastewater treatment
Wastewater treatment plants
Water treatment
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Summary:We applied fluorescence staining of Nile red, polymerase chain reaction (PCR), and carbon substrate utilization and pressure tolerance analysis to execute three-stage screening for potential polyhydroxyalkanoate (PHA) producers in the sludge samples of 21 large-scale wastewater treatment plants of city and industrial parks in Taiwan area. Total 35,429 colonies were grown on 196 plates, the screened 30 strains were subjected to 16S rRNA analysis, and 18 identified genera belonged to Proteobacteria (67%), Firmicutes (17%), and Actinomycetota (16%). The PHA accumulation results revealed that nine genera (50% of 18 screened) produced PHAs by limiting the nitrogen source and excess single carbon sources of glucose in an aerobic status. The PHA accumulation percentage was 1.44–58.77% at dry cell weight, and the theoretical yield from glucose was 0.52–58.76%. Our results indicate that our triple-screening method is promising for identifying a high biodiversity of PHA-accumulating bacteria from activated sludge for future industrial applications.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-023-03446-7