Synthesis and characterization of calcium-releasing elastomeric resin-based endodontic sealers

• Published in: Clinical oral investigations 2023-07, Vol.27 (7), p.3447-3456
• Main Authors: Ribeiro, Juliana S., Xavier, Samantha R., Cuevas Suárez, Carlos E., Pappen, Fernanda G., Piva, Evandro, Lund, Rafael G., Bottino, Marco C.
• Format: Article
• Language: English
• Subjects: Antimicrobial activity; Calcium hydroxide; Calcium phosphates; Dental caries; Dental equipment; Dental pulp; Dental restorative materials; Dentistry; Elastomers; Endodontics; Medicine; Nanotubes; Physicochemical properties; Sealing compounds; Toxicity; Tricalcium phosphate
• ISSN: 1436-3771; 1432-6981; 1436-3771
• DOI: 10.1007/s00784-023-04952-0

Objectives To evaluate the incorporation of halloysite nanotubes (HNTs) loaded with one of two calcium sources (i.e., calcium hydroxide/CaOH 2 or beta-tricalcium phosphate/β-TCP) on the physicochemical and biological properties of an experimental resin-based dual-cured endodontic sealer. Materials and methods HNTs were encapsulated with CaOH 2 or β-TCP at 10 wt.%. HNTs containing CaOH 2 or β-TCP were added into the experimental sealers at 50 wt.%. The control sealers were the calcium-free HNT-modified resin-based experimental sealer and AH Plus™, a commercially available endodontic sealer. Degree of conversion, setting time, flow, film thickness, radiopacity, dimensional stability, and calcium ions release were determined. Antibiofilm properties and cytocompatibility of the formulated sealers and commercial control were also evaluated. One and two-way ANOVA analysis followed by Tukey’s post hoc test was conducted to evaluate the effect of the independent variable on the evaluated properties. Results FTIR confirmed the encapsulation of calcium sources into HNTs. Regarding flow and film thickness, the values obtained from these sealers were in accordance with the specifications provided by ISO 6876. For radiopacity, AH Plus™ achieved the highest radiopacity ( p