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Novel function of a putative TaCOBL ortholog associated with cold response
The plant COBRA protein family plays an important role in secondary cell wall biosynthesis and the orientation of cell expansion. The COBRA gene family has been well studied in Arabidopsis thaliana , maize, rice, etc., but no systematic studies were conducted in wheat. In this study, the full-length...
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| Published in: | Molecular biology reports 2023-05, Vol.50 (5), p.4375-4384 |
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| container_title | Molecular biology reports |
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| creator | Liu, Fang-Fang Wan, Ying-Xiu Cao, Wen-Xin Zhang, Qi-Qi Li, Yao Li, Yan Zhang, Ping-Zhi Si, Hong-Qi |
| description | The plant COBRA protein family plays an important role in secondary cell wall biosynthesis and the orientation of cell expansion. The
COBRA
gene family has been well studied in
Arabidopsis thaliana
, maize, rice, etc., but no systematic studies were conducted in wheat. In this study, the full-length sequence of
TaCOBL
s was obtained by homology cloning from wheat, and a conserved motif analysis confirmed that
TaCOBL
s belonged to the COBRA protein family. qRT-PCR results showed that the
TaCOBL
transcripts were induced by abiotic stresses, including cold, drought, salinity, and abscisic acid (ABA). Two haplotypes of
TaCOBL-5B
(Hap5B-a and Hap5B-b), harboring one indel (---/TATA) in the 5′ flanking region (− 550 bp), were found on chromosome 5BS. A co-dominant marker, Ta5BF/Ta5BR, was developed based on the polymorphism of the two
TaCOBL-5B
haplotypes. Significant correlations between the two
TaCOBL-5B
haplotypes and cold resistance were observed under four environmental conditions. Hap5B-a, a favored haplotype acquired during wheat polyploidization, may positively contribute to enhanced cold resistance in wheat. Based on the promoter activity analysis, the Hap5B-a promoter containing a TATA-box was more active than that of Hap5B-b without the TATA-box under low temperature. Our study provides valuable information indicating that the
TaCOBL
genes are associated with cold response in wheat. |
| doi_str_mv | 10.1007/s11033-023-08297-5 |
| format | article |
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COBRA
gene family has been well studied in
Arabidopsis thaliana
, maize, rice, etc., but no systematic studies were conducted in wheat. In this study, the full-length sequence of
TaCOBL
s was obtained by homology cloning from wheat, and a conserved motif analysis confirmed that
TaCOBL
s belonged to the COBRA protein family. qRT-PCR results showed that the
TaCOBL
transcripts were induced by abiotic stresses, including cold, drought, salinity, and abscisic acid (ABA). Two haplotypes of
TaCOBL-5B
(Hap5B-a and Hap5B-b), harboring one indel (---/TATA) in the 5′ flanking region (− 550 bp), were found on chromosome 5BS. A co-dominant marker, Ta5BF/Ta5BR, was developed based on the polymorphism of the two
TaCOBL-5B
haplotypes. Significant correlations between the two
TaCOBL-5B
haplotypes and cold resistance were observed under four environmental conditions. Hap5B-a, a favored haplotype acquired during wheat polyploidization, may positively contribute to enhanced cold resistance in wheat. Based on the promoter activity analysis, the Hap5B-a promoter containing a TATA-box was more active than that of Hap5B-b without the TATA-box under low temperature. Our study provides valuable information indicating that the
TaCOBL
genes are associated with cold response in wheat.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-023-08297-5</identifier><identifier>PMID: 36944863</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Abscisic acid ; Abscisic Acid - metabolism ; Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Cell walls ; Chromosomes ; Cold ; Cold resistance ; Cold Temperature ; Conserved sequence ; Drought ; Environmental conditions ; Gene Expression Regulation, Plant - genetics ; Gene polymorphism ; Haplotypes ; Histology ; Homology ; Life Sciences ; Low temperature ; Morphology ; Original Article ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Polyploidy ; Promoter Regions, Genetic - genetics ; Stress, Physiological - genetics</subject><ispartof>Molecular biology reports, 2023-05, Vol.50 (5), p.4375-4384</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c370t-7755bdaf46c37b5ede0d621aa8306dab45edeccf3549d3adb922d503c09e12b53</cites><orcidid>0000-0002-6143-6866</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36944863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Fang-Fang</creatorcontrib><creatorcontrib>Wan, Ying-Xiu</creatorcontrib><creatorcontrib>Cao, Wen-Xin</creatorcontrib><creatorcontrib>Zhang, Qi-Qi</creatorcontrib><creatorcontrib>Li, Yao</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Zhang, Ping-Zhi</creatorcontrib><creatorcontrib>Si, Hong-Qi</creatorcontrib><title>Novel function of a putative TaCOBL ortholog associated with cold response</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>The plant COBRA protein family plays an important role in secondary cell wall biosynthesis and the orientation of cell expansion. The
COBRA
gene family has been well studied in
Arabidopsis thaliana
, maize, rice, etc., but no systematic studies were conducted in wheat. In this study, the full-length sequence of
TaCOBL
s was obtained by homology cloning from wheat, and a conserved motif analysis confirmed that
TaCOBL
s belonged to the COBRA protein family. qRT-PCR results showed that the
TaCOBL
transcripts were induced by abiotic stresses, including cold, drought, salinity, and abscisic acid (ABA). Two haplotypes of
TaCOBL-5B
(Hap5B-a and Hap5B-b), harboring one indel (---/TATA) in the 5′ flanking region (− 550 bp), were found on chromosome 5BS. A co-dominant marker, Ta5BF/Ta5BR, was developed based on the polymorphism of the two
TaCOBL-5B
haplotypes. Significant correlations between the two
TaCOBL-5B
haplotypes and cold resistance were observed under four environmental conditions. Hap5B-a, a favored haplotype acquired during wheat polyploidization, may positively contribute to enhanced cold resistance in wheat. Based on the promoter activity analysis, the Hap5B-a promoter containing a TATA-box was more active than that of Hap5B-b without the TATA-box under low temperature. Our study provides valuable information indicating that the
TaCOBL
genes are associated with cold response in wheat.</description><subject>Abscisic acid</subject><subject>Abscisic Acid - metabolism</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell walls</subject><subject>Chromosomes</subject><subject>Cold</subject><subject>Cold resistance</subject><subject>Cold Temperature</subject><subject>Conserved sequence</subject><subject>Drought</subject><subject>Environmental conditions</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Gene polymorphism</subject><subject>Haplotypes</subject><subject>Histology</subject><subject>Homology</subject><subject>Life Sciences</subject><subject>Low temperature</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Polyploidy</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Stress, Physiological - genetics</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kM9PwyAUx4nR6Jz-Ax4MiRcv1QeU0h518WcWd5lnQoFuXboyoZ3xv5e5qYkHD-QF-Lzve_kgdEbgigCI60AIMJYAjSenhUj4HhoQLliSFiLfRwNgQJI05-QIHYewAICUCH6IjlhWpGmesQF6fnFr2-Cqb3VXuxa7Ciu86jvV1WuLp2o0uR1j57u5a9wMqxCcrlVnDX6vuznWrjHY27BybbAn6KBSTbCnuzpEr_d309FjMp48PI1uxolmArpECM5Lo6o0i_eSW2PBZJQolTPIjCrTzZPWFeNpYZgyZUGp4cA0FJbQkrMhutzmrrx7623o5LIO2jaNaq3rg6QiLwShlJGIXvxBF673bdxO0hxEZCIaKbqltHcheFvJla-Xyn9IAnJjWm5Ny2hafpmWmy3Od9F9ubTmp-VbbQTYFgjxq51Z_zv7n9hPseyInA</recordid><startdate>20230501</startdate><enddate>20230501</enddate><creator>Liu, Fang-Fang</creator><creator>Wan, Ying-Xiu</creator><creator>Cao, Wen-Xin</creator><creator>Zhang, Qi-Qi</creator><creator>Li, Yao</creator><creator>Li, Yan</creator><creator>Zhang, Ping-Zhi</creator><creator>Si, Hong-Qi</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6143-6866</orcidid></search><sort><creationdate>20230501</creationdate><title>Novel function of a putative TaCOBL ortholog associated with cold response</title><author>Liu, Fang-Fang ; Wan, Ying-Xiu ; Cao, Wen-Xin ; Zhang, Qi-Qi ; Li, Yao ; Li, Yan ; Zhang, Ping-Zhi ; Si, Hong-Qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-7755bdaf46c37b5ede0d621aa8306dab45edeccf3549d3adb922d503c09e12b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Abscisic acid</topic><topic>Abscisic Acid - metabolism</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell walls</topic><topic>Chromosomes</topic><topic>Cold</topic><topic>Cold resistance</topic><topic>Cold Temperature</topic><topic>Conserved sequence</topic><topic>Drought</topic><topic>Environmental conditions</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Gene polymorphism</topic><topic>Haplotypes</topic><topic>Histology</topic><topic>Homology</topic><topic>Life Sciences</topic><topic>Low temperature</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Polyploidy</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Stress, Physiological - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Fang-Fang</creatorcontrib><creatorcontrib>Wan, Ying-Xiu</creatorcontrib><creatorcontrib>Cao, Wen-Xin</creatorcontrib><creatorcontrib>Zhang, Qi-Qi</creatorcontrib><creatorcontrib>Li, Yao</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Zhang, Ping-Zhi</creatorcontrib><creatorcontrib>Si, Hong-Qi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest - 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Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Fang-Fang</au><au>Wan, Ying-Xiu</au><au>Cao, Wen-Xin</au><au>Zhang, Qi-Qi</au><au>Li, Yao</au><au>Li, Yan</au><au>Zhang, Ping-Zhi</au><au>Si, Hong-Qi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel function of a putative TaCOBL ortholog associated with cold response</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2023-05-01</date><risdate>2023</risdate><volume>50</volume><issue>5</issue><spage>4375</spage><epage>4384</epage><pages>4375-4384</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>The plant COBRA protein family plays an important role in secondary cell wall biosynthesis and the orientation of cell expansion. The
COBRA
gene family has been well studied in
Arabidopsis thaliana
, maize, rice, etc., but no systematic studies were conducted in wheat. In this study, the full-length sequence of
TaCOBL
s was obtained by homology cloning from wheat, and a conserved motif analysis confirmed that
TaCOBL
s belonged to the COBRA protein family. qRT-PCR results showed that the
TaCOBL
transcripts were induced by abiotic stresses, including cold, drought, salinity, and abscisic acid (ABA). Two haplotypes of
TaCOBL-5B
(Hap5B-a and Hap5B-b), harboring one indel (---/TATA) in the 5′ flanking region (− 550 bp), were found on chromosome 5BS. A co-dominant marker, Ta5BF/Ta5BR, was developed based on the polymorphism of the two
TaCOBL-5B
haplotypes. Significant correlations between the two
TaCOBL-5B
haplotypes and cold resistance were observed under four environmental conditions. Hap5B-a, a favored haplotype acquired during wheat polyploidization, may positively contribute to enhanced cold resistance in wheat. Based on the promoter activity analysis, the Hap5B-a promoter containing a TATA-box was more active than that of Hap5B-b without the TATA-box under low temperature. Our study provides valuable information indicating that the
TaCOBL
genes are associated with cold response in wheat.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>36944863</pmid><doi>10.1007/s11033-023-08297-5</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-6143-6866</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Abscisic acid Abscisic Acid - metabolism Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Cell walls Chromosomes Cold Cold resistance Cold Temperature Conserved sequence Drought Environmental conditions Gene Expression Regulation, Plant - genetics Gene polymorphism Haplotypes Histology Homology Life Sciences Low temperature Morphology Original Article Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - genetics Polyploidy Promoter Regions, Genetic - genetics Stress, Physiological - genetics |
| title | Novel function of a putative TaCOBL ortholog associated with cold response |
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