Macrovascular and microvascular type 2 diabetes complications are interrelated in a mouse model

• Published in: Journal of diabetes and its complications 2023-05, Vol.37 (5), p.108455-108455, Article 108455
• Main Authors: Cifuentes-Mendiola, S.E., Solís-Suarez, D.L., Martínez-Davalos, A., García-Hernández, A.L.
• Format: Article
• Language: English
• Subjects: Age; Animal research; Animals; Body mass index; Collagen; Diabetes; Diabetes complications; Diabetes Complications - complications; Diabetes Mellitus, Type 2; Diabetic neuropathy; Disease; Disease Models, Animal; Exocrine glands; Glucose; Hyperglycaemia; Hyperglycemia; Insulin Resistance; Kidneys; Liver; Male; Metabolism; Mice; Mice, Inbred C57BL; Mortality; Pathophysiology; Type two diabetes
• ISSN: 1056-8727; 1873-460X
• DOI: 10.1016/j.jdiacomp.2023.108455

Evaluate the development of multiple complications, their interactions, and common mechanisms in the same individual with T2D. 4-week-old male C57BL/6J mice were divided into: control (n = 6) and T2D (n = 6). T2D was induced through a high-carbohydrate-diet and low doses of streptozotocin. T2D was validated by metabolic parameters. Diabetic neuropathy was evaluated by mechanical and thermal sensitivity tests. We performed a histopathological analysis of the heart, kidney, liver, and parotid salivary glands and changes in bone microarchitecture by μCT. We calculated the relative risk (RR), odd ratios (OR) and Pearson correlation coefficients between the different complications and metabolic features. T2D mice have cardiomyopathy, neuropathy, nephropathy, liver steatosis and fibrosis, structural damage in parotid salivary glands, and bone porosity. RR analysis shows that all complications are interrelated by hyperglycaemia, insulin resistance, obesity, and systemic inflammation. T2D mice develop multiple complications simultaneously, which are related to each other, and this is associated with metabolic alterations. Our findings open up new approaches for the study and new therapeutic approaches of the pathophysiology of T2D and its complications. •Type 2 Diabetes complications are interrelated in mouse.•Type 2 Diabetes complications are interrelated by metabolic alterations.•Cardiomyopathy, neuropathy, nephropathy, and hepatopathy are interrelated in T2D mouse.