Staphylococcus aureus nasal colonization level and intracellular reservoir: a prospective cohort study

• Published in: European journal of clinical microbiology & infectious diseases 2023-05, Vol.42 (5), p.621-629
• Main Authors: Rigaill, Josselin, Gavid, Marie, Fayolle, Martin, Morgene, Mohamed Fedy, Lelonge, Yann, Grattard, Florence, Pozzetto, Bruno, Crépin, Adeline, Prades, Jean-Michel, Laurent, Frédéric, Botelho-Nevers, Elisabeth, Berthelot, Philippe, Verhoeven, Paul O.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; Carrier State - epidemiology; Carrier State - microbiology; Cohort analysis; Colonization; Endoscopy; Genotypes; Genotyping; Health risks; Host-pathogen interactions; Humans; Internal Medicine; Intracellular; Medical Microbiology; Nasal Cavity - microbiology; Nose; Nose - microbiology; Original Article; Pathogens; Penicillin; Prospective Studies; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus aureus - genetics; Vestibules
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/s10096-023-04591-z

Staphylococcus aureus is a major pathogen in humans. The nasal vestibule is considered as the main reservoir of S. aureus. However, even though the nasal cavity may also be colonized by S. aureus, the relationships between the two sites are still unclear. We conducted a prospective study in humans to assess the S. aureus colonization profiles in the vestibule and nasal cavity, and to investigate the presence of intracellular S. aureus in the two sites. Patients undergoing ear, nose, and throat surgery were swabbed during endoscopy to determine S. aureus nasal load, genotype, and presence of intracellular S. aureus . Among per-operative samples from 90 patients, the prevalence of S. aureus carriage was 32.2% and 33.3% in the vestibule and the nasal cavity, respectively. The mean S. aureus load was 4.10 and 4.25 log 10 CFU/swab for the nasal vestibule and nasal cavity, respectively ( P > 0.05). Genotyping of S. aureus revealed that all nasal strains isolated from a given individual belong to the same clonal complex and spa-type. An intracellular carriage was observed in 5.6% of the patients, all of whom exhibited a S. aureus vestibule load higher than 3 log 10 CFU/swab. An intracellular niche was observed in the vestibule as well as in the nasal cavity. In conclusion, the nasal cavity was also found to be a major site of S. aureus carriage in humans and should draw attention when studying host-pathogen interactions related to the risk of infection associated with colonization.