Immunochemical identification of the mammalian peptide hormone obestatin from tea plant (Camellia sinensis)

This study determines obestatin-like substances from the young shoots of the tea plant [Camellia sinensis (L.) O. Kuntze (Theaceae)]. Proteins were extracted from the vegetative tea leaves using the QB (Quick Buffer) buffer as an extraction buffer. Obestatin-like substances in tea extract were inves...

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Published in:Pakistan journal of pharmaceutical sciences 2023-01, Vol.36 (1), p.199-204
Main Authors: Calapoglu, Mustafa, Temizyurek, Iftihar, Ilhan, Ilter, Cicek, Emine, Kumbul Doguc, Duygu, Sahin, Ugur
Format: Article
Language:English
Subjects:
Animals
Camellia sinensis - chemistry
Ghrelin - analysis
Ghrelin - metabolism
Humans
Mammals
Plant Extracts - analysis
Plant Leaves - chemistry
Plant Proteins - analysis
Tea - chemistry
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Summary:This study determines obestatin-like substances from the young shoots of the tea plant [Camellia sinensis (L.) O. Kuntze (Theaceae)]. Proteins were extracted from the vegetative tea leaves using the QB (Quick Buffer) buffer as an extraction buffer. Obestatin-like substances in tea extract were investigated using an indirect home-made enzyme-linked immunosorbent assay (ELISA). Human obestatin-like immunoreactive substances from tea extract were isolated and characterized by tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) and immunoblotting techniques. Immunochemical results showed that there are strong human obestatin-like immunoreactive substances (0.048±0.0064ng/mg protein) in vegetative tea leaves. This finding was completely unexpected since this hormone was considered to be present solely in animals. Furthermore, a single obestatin-like immunoreactive protein band of 13kDa was identified by tricine-SDS-PAGE and Western blotting of extract of vegetative tea leaf proteins. Present investigation is the first report of presence of obestatin-like immunoreactive substances in plants. It is concluded that obestatin-like bioactive peptides derived from plants can affect gastrointestinal tract structures as endogenous obestatin does and hence play a role in appetite regulation and body weight gain.
ISSN:1011-601X
DOI:10.36721/PJPS.2023.36.1.REG.199-204.1