Conductive materials enhance microbial salt-tolerance in anaerobic digestion of food waste: Microbial response and metagenomics analysis

• Published in: Environmental research 2023-06, Vol.227, p.115779-115779, Article 115779
• Main Authors: Li, Jianhao, Xu, Xiaofeng, Chen, Cong, Xu, Linji, Du, Zexuan, Gu, Li, Xiang, Ping, Shi, Dezhi, Huangfu, Xiaoliu, Liu, Feng
• Format: Article
• Language: English
• Subjects: Anaerobic digestion; Anaerobiosis; Bioreactors; Charcoal; Conductive materials; Direct interspecies electron transfer; Ferrosoferric Oxide; Food; Graphite; Metabolic reconstruction; Metagenomics; Methane; Refuse Disposal; Salinity stress; Salt Tolerance; Sewage
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2023.115779

Previous studies have shown that high salinity environments can inhibit anaerobic digestion (AD) of food waste (FW). Finding ways to alleviate salt inhibition is important for the disposal of the growing amount of FW. We selected three common conductive materials (powdered activated carbon, magnetite, and graphite) to understand their performance and individual mechanisms that relieve salinity inhibition. Digester performances and related enzyme parameters were compared. Our data revealed that under normal and low salinity stress conditions, the anaerobic digester ran steady without significant inhibitions. Further, the presence of conductive materials promoted conversion rate of methanogenesis. This promotion effect was highest from magnetite > powdered activated carbon (PAC) > graphite. At 1.5% salinity, PAC and magnetite are beneficial in maintaining high methane production efficiency while control and the graphite added digester acidified and failed rapidly. Additionally, metagenomics and binning were used to analyze the metabolic capacity of the microorganisms. Some species enriched by PAC and magnetite possessed higher cation transport capacities and were to accumulate compatible solutes. PAC and magnetite promoted direct interspecies electron transference (DIET) and syntrophic oxidation of butyrate and propionate. Also, the microorganisms had more energy available to cope with salt inhibition in the PAC and magnetite added digesters. Our data imply that the promotion of Na+/H+ antiporter, K+ uptake, and osmoprotectant synthesis or transport by conductive materials may be crucial for their proliferation in highly stressful environments. These findings will help to understand the mechanisms of alleviate salt inhibition by conductive materials and help to recover methane from high-salinity FW. [Display omitted] •Powdered activated carbon and magnetite enhance digester stability.•Potentially salt-tolerant bacteria were enriched by PAC and magnetite.•Direct interspecies electron transfer were enhanced by conductive materials.•Energy metabolism and enzyme activity were enhanced by PAC and magnetite.•PAC and magnetite improved the abundance of salt-tolerance genes.