CDK10, CDK11, FOXO1, and FOXO3 Gene Expression in Alzheimer’s Disease Encephalic Samples

• Published in: Cellular and molecular neurobiology 2023-08, Vol.43 (6), p.2953-2962
• Main Authors: Fredi, Bruno Mari, De Labio, Roger Willian, Rasmussen, Lucas Trevizani, Chagas, Eduardo Federighi Baisi, Chen, Elizabeth Suchi, Turecki, Gustavo, Smith, Marilia de Arruda Cardoso, Payão, Spencer Luiz Marques
• Format: Article
• Language: English
• Subjects: Aged; Alzheimer Disease - metabolism; Alzheimer's disease; Amyloid beta-Peptides - metabolism; Biomedical and Life Sciences; Biomedicine; Brain - metabolism; Cell Biology; Cerebellum; Cortex (auditory); Cyclin-Dependent Kinases - genetics; Cyclin-Dependent Kinases - metabolism; Forkhead Box Protein O1 - genetics; Forkhead Box Protein O1 - metabolism; Forkhead Box Protein O3 - genetics; FOXO1 protein; FOXO3 protein; Gene Expression; Genetic factors; Humans; Inflammation; Neurobiology; Neurodegenerative diseases; Neurofibrillary tangles; Neurofibrillary Tangles - metabolism; Neurosciences; Original Research; Statistical analysis; β-Amyloid
• ISSN: 0272-4340; 1573-6830
• DOI: 10.1007/s10571-023-01341-9

Alzheimer’s disease (AD) is a progressive neuroinflammatory and neurodegenerative disorder that affects different regions of the brain. Its pathophysiology includes the accumulation of β-amyloid protein, formation of neurofibrillary tangles, and inflammatory processes. Genetic factors are involved in the onset of AD, but they are not fully elucidated. Identification of gene expression in encephalic tissues of patients with AD may help elucidate its development. Our objectives were to characterize and compare the gene expression of CDK10, CDK11, FOXO1, and FOXO3 in encephalic tissue samples from AD patients and elderly controls, from the auditory cortex and cerebellum. RT-qPCR was used on samples from 82 individuals (45 with AD and 37 controls). We observed a statistically significant increase in CDK10 ( p  = 0.029*) and CDK11 ( p  = 0.048*) gene expression in the AD group compared to the control, which was most evident in the cerebellum. Furthermore, the Spearman test demonstrated the presence of a positive correlation of gene expression both in the auditory cortex in the AD group ( r  = 0.046/ p  = 0.004) and control group ( r  = 0.454/ p  = 0.005); and in the cerebellum in the AD group ( r  = 0.654 / p