The antioxidant defence of Dekkera bruxellensis against hydrogen peroxide and its relationship to nitrate metabolism

• Published in: Journal of applied microbiology 2023-04, Vol.134 (4)
• Main Authors: Xavier, Mariana Rodrigues, Teles, Gilberto Henrique, de Carvalho, Jennyfer Martins, Vieira, Leucio Duarte, Pita, Will de Barros, Antonio de Morais, Marcos
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Dekkera - genetics; Dekkera - metabolism; Fermentation; Hydrogen Peroxide - metabolism; Nitrates - metabolism; Nitrogen - metabolism; Saccharomyces cerevisiae - metabolism
• ISSN: 1365-2672; 1365-2672
• DOI: 10.1093/jambio/lxad065

The yeast Dekkera bruxellensis is a Crabtree-positive yeast that tends towards the oxidative/respiratory metabolism in aerobiosis. However, it is more sensitive to H2O2 than Saccharomyces cerevisiae. In order to investigate this metabolic paradox, the present work aimed to uncover the biological defence mechanism used by this yeast to tolerate the presence of exogenous H2O2. Growth curves and spot tests were performed to establish the values of minimal inhibitory concentration and minimal biocidal concentration of H2O2 for different combinations of carbon and nitrogen sources. Cells in exponential growth phase in different culture conditions were used to measure superoxide and thiols [protein (PT) and non-PT], enzyme activities and gene expression. The combination of glutathione peroxidase (Gpx) and sulfhydryl-containing PT formed the preferred defence mechanism against H2O2, which was more efficiently active under respiratory metabolism. However, the action of this mechanism was suppressed when the cells were metabolizing nitrate (NO3). These results were relevant to figure out the fitness of D. bruxellensis to metabolize industrial substrates containing oxidant molecules, such as molasses and plant hydrolysates, in the presence of a cheaper nitrogen source such as NO3.