Toxicogenic fungal profile, Ochratoxin A exposure and cancer risk characterization through maize (Zea mays) consumed by different age populations in the Volta region of Ghana

• Published in: Toxicon (Oxford) 2023-04, Vol.226, p.107085-107085, Article 107085
• Main Authors: Kortei, Nii Korley, Oman Ayiku, Peter, Nsor-Atindana, John, Owusu Ansah, Leslie, Wiafe-Kwagyan, Michael, Kyei-Baffour, Vincent, Kottoh, Isaac Delali, Odamtten, George Tawia
• Format: Article
• Language: English
• Subjects: Agar; Cancer; Food Contamination - analysis; Fungi; Ghana; Humans; Maize; Mycotoxins - analysis; Neoplasms; Ochratoxin A; Ochratoxins - analysis; Ochratoxins - toxicity; Risk assessment; Toxicogenic fungi; Zea mays - chemistry; Zea mays - microbiology
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2023.107085

Maize (Zea mays) is an important staple food crop for the majority of Ghanaians. Maize is mostly contaminated by fungal species and particularly mycotoxins. This work aimed to identify and quantify the incidence of fungal infection and exposure to Ochratoxin A (OTA) as well as the health risk characterization in different age populations due to maize consumption in the Volta region. Maize samples were plated on Dichloran Rose Bengal Chloramphenicol (DRBC) agar, and Oxytetracycline Glucose Yeast Extract (OGYE) agar. All media were prepared in accordance with the manufacturers’ instructions. The plates were incubated at 28 ± 2 °C for 5–7 days. High-Performance Liquid Chromatography connected to a fluorescence detector (HPLC-FLD) was used to analyze the ochratoxin A (OTA) levels in maize. Cancer risk assessments were also conducted using models prescribed by the Joint FAO/WHO Expert Committee on Additives (JECFA). The maize samples collected from the Volta region contained fungal population between the range of 3.08–4.58 log10 CFU/g. Eight (8) genera were recorded belonging to Aspergillus, Trichoderma, Penicillium, Fusarium, Saccharomyces, Mucor, Rhodotorula and Rhizopus. The species diversity includes A. flavus, A. niger, T. harzianum, P. verrucosum, F. oxysporum, Yeast, F. verticillioides, Rhodotorulla sp, A. fumigatus, R. stolonifer, M. racemosus species. Additionally, the ochratoxins level contained in the samples were very noteworthy and ranged from 1.22 to 28.17 μg/kg. Cancer risk assessments of OTA produced outcomes also ranged between 2.15 and 524.54 ng/kg bw/day, 0.03–8.31, 0.0323, and 0.07–16.94 for cases/100,000 person/yr for Estimated Daily Intake (EDI), Margin of Exposure (MOE), Average Potency, and Cancer Risks respectively for all age categories investigated. There was very high mycoflora load on the maize sampled from the Volta region, likewise the range of mycotoxins present in the maize grains, suggesting the potential to pose some adverse health effects with the populace of the Volta region. [Display omitted] •Maize samples were contaminated with 13 toxicogenic fungal species.•The ochratoxin A (OTA) levels contained in the maize samples were significant and ranged from 1.22 to 28.17 μg/kg.•Exposure to OTA and its vulnerability, principally results from the consumption of maize contaminated with toxicogenic fungi.•Risk assessment on raw maize consumption, revealed a public health concern and an importance for risk management.