Expression analysis of Rho GTPase-related lncRNAs in breast cancer

• Published in: Pathology, research and practice research and practice, 2023-04, Vol.244, p.154429-154429, Article 154429
• Main Authors: Nicknam, Amir, Khojasteh Pour, Sahar, Hashemnejad, Mohammad Amin, Hussen, Bashdar Mahmud, Safarzadeh, Arash, Eslami, Solat, Taheri, Mohammad, Ghafouri-Fard, Soudeh, Jamali, Elena
• Format: Article
• Language: English
• Subjects: Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Carcinogenesis - genetics; Cell Line, Tumor; DANCR; Female; Gene Expression Regulation, Neoplastic - genetics; Humans; LncRNA; MicroRNAs - genetics; NORAD; NRAV; RAD51-AS1; rho GTP-Binding Proteins - genetics; rho GTP-Binding Proteins - metabolism; Rho GTPase; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism
• ISSN: 0344-0338; 1618-0631
• DOI: 10.1016/j.prp.2023.154429

The Rho GTPases have prominent roles in cell cycle transition and cell migration. Some members of this family have been found to be mutated in cancers. Moreover, alterations in expression levels and/or activity of these proteins have been reported in many types of cancers. Thus, Rho GTPases are involved in the carcinogenesis. Rho GTPases regulate growth, motility, invasiveness and metastatic ability of breast cancer cells. Long non-coding RNAs (lncRNAs) have been revealed to exert significant effect in the regulation of these proteins via direct routes or through sequestering microRNAs that inhibit Rho GTPases. We aimed to assess expression levels of four Rho GTPase-related lncRNAs, namely NORAD, RAD51-AS1, NRAV and DANCR in breast cancer samples versus non-cancerous specimens from the same individuals. Expression levels of NORAD were shown to be elevated in tumoral tissues compared with non-tumoral tissues (Expression ratio (95% CI)= 5.85 (3.16–10.83), Standard error of mean (SEM)= 0.44, P value