Differential effects of continuous and intermittent daytime food deprivation periods on metabolism and reproductive performance in diurnal zebra finches

• Published in: Hormones and behavior 2023-06, Vol.152, p.105353-105353, Article 105353
• Main Authors: Prabhat, Abhilash, Buniyaadi, Amaan, Bhardwaj, Sanjay Kumar, Kumar, Vinod
• Format: Article
• Language: English
• Subjects: Animals; Female; Finches; Food; Food Deprivation; Gene expression; Hypothalamus; Hypothalamus - metabolism; Liver; Metabolism; Oviposition; Reproduction; Zebra finch
• ISSN: 0018-506X; 1095-6867
• DOI: 10.1016/j.yhbeh.2023.105353

We investigated whether food availability effects on metabolism and reproduction are the result of the sum effect of daily feeding (food availability) and starvation (food deprivation) periods. Adult zebra finches were paired and subjected to a time-restricted feeding (TRF) regimen consisting of continuous and intermittent daytime food deprivation periods. Birds were given food during the 12-h day for a total of 4-h in the evening (1 *4-h, hour 8–12), or in 2 splits of 2 h each (2 * 2-h) or 4 splits 1 h each (4 * 1-h), with controls on food ad libitum, until they had the first egg clutch. TRF caused significant changes in hepatic expression of metabolism-associated sirt1, egr1, pparα and foxo1 genes despite no difference in the food intake, body mass and blood glucose levels. Importantly, TRF resulted in a significant reduction in plasma testosterone and estradiol levels, delayed nest-building and egg laying, and reduced clutch size. Concurrently, under TRF regimes, we found a significantly lower expression of th and mtr genes linked with motivation and affiliation (but not of dio2, dio3, gnrh1 and gnih genes linked with gonadal maturation) in the hypothalamus, and of star and hook 1 genes in the testes and star, cyp19 and erα genes in the ovary. These results demonstrate the importance of daily food deprivation times on the metabolism and reproduction, and suggest a possible provisioning of energy available from daily feeding for the maintenance of body condition at the expense of reproduction performance in diurnal animals. •Time-restricted feeding (TRF) did not affect daily food intake and body condition.•TRF affected reproductive health (low T and E2) and performance (fewer eggs laid).•Altered effects on gene expressions in the liver, hypothalamus and gonads•TRF affected expression of genes linked to metabolism, motivation and affiliation.•Differential effects of continuous and intermittent daytime starvation periods