Synthesis of lysinonorleucine and mass spectrometric analysis of lysinonorleucine and merodesmosine in bovine ligament and eggshell membrane

Elastin is an important extracellular matrix protein that contributes to the elasticity of cells, tissues, and organs. Although crosslinking amino acids such as desmosine and isodesmosine have been identified in elastin, details regarding the structure remain unclear. In this study, an elastin cross...

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Bibliographic Details
Published in:Archives of biochemistry and biophysics 2023-05, Vol.740, p.109585-109585, Article 109585
Main Authors: Oishi, Kaho, Mori, Natsuki, Anzawa, Riki, Usuki, Toyonobu
Format: Article
Language:English
Subjects:
Animals
Bovine ligament
Cattle
Desmosine - metabolism
Egg Shell - chemistry
Egg Shell - metabolism
Eggshell membrane
Elastin - chemistry
Ligaments - chemistry
Ligaments - metabolism
Lysinonorleucine
Merodesmosine
Tandem mass spectrometry
Total synthesis
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Summary:Elastin is an important extracellular matrix protein that contributes to the elasticity of cells, tissues, and organs. Although crosslinking amino acids such as desmosine and isodesmosine have been identified in elastin, details regarding the structure remain unclear. In this study, an elastin crosslinker, lysinonorleucine, was chemically synthesized and detected in hydrolyzed bovine ligament and eggshell membrane samples utilizing tandem mass spectrometry. Merodesmosine, another crosslinker of elastin, was also measured in the same samples using the same analytical method. The resulting data should aid in the elucidating the crosslinking structure of elastin and eggshell membranes. [Display omitted]
ISSN:0003-9861
1096-0384
DOI:10.1016/j.abb.2023.109585