LncRNA PVT1 delays skin photoaging by sequestering miR-551b-3p to release AQP3 expression via ceRNA mechanism

• Published in: Apoptosis (London) 2023-06, Vol.28 (5-6), p.912-924
• Main Authors: Tang, Hua, Xiong, Qi, Yin, Ming, Feng, Hao, Yao, Fang, Xiao, Xiao, Hu, Feng, Liao, Yangying
• Format: Article
• Language: English
• Subjects: Analysis; Apoptosis - genetics; Aquaporin 3; Aquaporin 3 - genetics; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cancer Research; Cell Biology; Cell cycle; Cholecystokinin; Collagen; Deactivation; Fibroblasts; Flow cytometry; Humans; MAP kinase; Mediation; MicroRNA; MicroRNAs; MicroRNAs - metabolism; miRNA; mRNA; Non-coding RNA; Oncology; p38 Mitogen-Activated Protein Kinases - metabolism; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Senescence; Sequestering; Signal transduction; Signaling; Skin; Skin Aging - genetics; Virology
• ISSN: 1360-8185; 1573-675X
• DOI: 10.1007/s10495-023-01834-4

Understanding human skin photoaging requires in-depth knowledge of the molecular and functional mechanisms. Human dermal fibroblasts (HDFs) gradually lose their ability to produce collagen and renew intercellular matrix with aging. Therefore, our study aims to reveal the mechanistic actions of a novel ceRNA network in the skin photoaging by regulating HDF activities. Photoaging-related genes were obtained in silico , followed by GO and KEGG enrichment analyses. Differentially expressed lncRNAs and miRNAs were screened from the GEO database to construct the ceRNA co-expression network. In skin photoaging samples, PVT1 and AQP3 were poorly expressed, while miR-551b-3p was highly expressed. The relationships among the lncRNA, miRNA and mRNA were explored through the ENCORI database and dual luciferase reporter assay. Mechanistically, PVT1 could sequester miR-551b-3p to upregulate the expression of AQP3, which further inactivated the ERK/p38 MAPK signaling pathway. HDFs were selected to construct an in vitro cell skin photoaging model, where the senescence, cell cycle distribution and viability of young and senescent HDFs were detected by SA-β-gal staining, flow cytometry and CCK-8 assay. In vitro cell experiments confirmed that overexpression of PVT1 or AQP3 enhanced viability of young and senescent HDFs and inhibited HDF senescence, while miR-551b-3p upregulation counteracted the effect of PVT1. In conclusion, PVT1-driven suppression of miR-551b-3p induces AQP3 expression to inactivate the ERK/p38 MAPK signaling pathway, thereby inhibiting HDF senescence and ultimately delaying the skin photoaging.