Bifunctional Single-Molecular Fluorescent Probe: Visual Detection of Mitochondrial SO2 and Membrane Potential

Mitochondrial membrane potential (MMP) and sulfur dioxide (SO2) significantly affect the mitochondrial state. In this work, TC-2 and TC-8 were constructed through side-chain engineering, in which TC-2 bearing the poorer hydrophobicity could localize on mitochondria better. Interestingly, short-wave...

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Published in:Analytical chemistry (Washington) 2023-04, Vol.95 (15), p.6287-6294
Main Authors: Ai, Wenming, Bu, Yingcui, Huang, Houshi, Wang, Junjun, Ren, Mengjuan, Deng, Yu, Zhu, Yicai, Wang, Sen, Yu, Zhi-Peng, Zhou, Hongping
Format: Article
Language:English
Subjects:
Chemistry
Deoxyribonucleic acid
DNA
Emission
Fluorescence
Fluorescent indicators
Hydrophobicity
Membrane potential
Membranes
Mitochondria
Oxidative stress
Reactive oxygen species
Short wave radiation
Sulfur
Sulfur dioxide
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Summary:Mitochondrial membrane potential (MMP) and sulfur dioxide (SO2) significantly affect the mitochondrial state. In this work, TC-2 and TC-8 were constructed through side-chain engineering, in which TC-2 bearing the poorer hydrophobicity could localize on mitochondria better. Interestingly, short-wave emission was captured due to the sensitive response of TC-2 to SO2 (LOD = 13.8 nM). Meanwhile, the probe could bind with DNA, presenting enhanced long-wave emission. Encouragingly, TC-2 could migrate from mitochondria to the nucleus when MMP was decreased, accompanied by the increase of fluorescence lifetime (9-fold). Hence, TC-2 could be used for dual-channel monitoring of mitochondrial SO2 and MMP, which showed a completely different pathway from the commercial MMP detectors JC-1/JC-10. The cellular experiments showed that MMP was gradually decreased due to reactive oxygen species-triggered oxidative stress, and the SO2 level was up-regulated simultaneously. Overall, this work proposed a new method to investigate and diagnose the mitochondrial-related diseases.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.2c05021