Plasticizer phthalate esters degradation with a laccase from Trametes versicolor: effects of TEMPO used as a mediator and estrogenic activity removal

• Published in: Biodegradation (Dordrecht) 2023-10, Vol.34 (5), p.431-444
• Main Authors: Wang, Dan, Zhu, Ruofei, Lou, Jiangfei, Baek, Nawon, Fan, Xuerong
• Format: Article
• Language: English
• Subjects: Aluminum; Analysis; Aquatic Pollution; Biodegradation; Biomedical and Life Sciences; Calcium ions; Chain branching; Chemicals; Copper; Degradation; Drinking water; Energy consumption; Enzymes; Esters; Estrogenic activity; Feasibility studies; Geochemistry; Laccase; Life Sciences; Metal concentrations; Metal ions; Metals; Microbiology; Organic compounds; Original Paper; Oxidases; Oxidation; Phthalate esters; Phthalates; Phthalic acid; Pollutants; Soil Science & Conservation; System effectiveness; Terrestrial Pollution; Trametes versicolor; Waste Management/Waste Technology; Waste Water Technology; Water Management; Water Pollution Control; Xenoestrogens
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1007/s10532-023-10030-9

Phthalate esters (PAEs) are toxic and persistent chemicals that are ubiquitous in the environment and have attracted worldwide attention due to their threats to the environment and human health. Dimethyl phthalate (DMP) is a relatively simple structure and one of the most observed PAEs in the environment. This study investigated the degradation of the DMP using Trametes versicolor laccase and its laccase-mediator systems. The degradation effect of laccase alone on DMP was poor, while the laccase-mediator systems can effectively enhance the degradation efficiency. Within 24 h, 45% of DMP (25 mg/L) was degraded in the presence of 0.8 U/mL laccase and 0.053 mM 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO). A certain concentration (1 mM) of metal ions Al 3+ , Cu 2+ or Ca 2+ can positively promote DMP degradation with the laccase-TEMPO system. Moreover, the structure of PAEs also had a great influence on the degradation efficiency. Higher degradation efficiencies were observed when incubating PAEs with short alkyl side chains by the laccase-TEMPO system compared to that with long alkyl side chains. Additionally, the branched-chain PAEs had a better degradation effect than the straight-chain. The estrogenic activity of the DMP solution after reaction was much smaller than that of the original solution. Finally, transformation products ortho-hydroxylated DMP and phthalic acid were identified by GC–MS and the possible degradation pathway was proposed. This study verifies the feasibility of the laccase-TEMPO system to degrade PAEs and provides a reference for exploring more potential value of laccase.