Chia (Salvia hispanica L.) can directly suppress basic ovarian cell functions in two farm animal species and protect ovarian cells from the proliferation‐stimulating influence of xylene

• Published in: Reproduction in domestic animals 2023-06, Vol.58 (6), p.823-832
• Main Authors: Tarko, Adam, Štochmaľová, Aneta, Fabová, Zuzana, Harrath, Abdel Halim, Sirotkin, Alexander V.
• Format: Article
• Language: English
• Subjects: Animal species; Animals; Animals, Domestic; Apoptosis; Cattle; Cell Proliferation; Cells, Cultured; chia; Contaminants; Environmental effects; Female; Food plants; Functional foods & nutraceuticals; Functionals; Granulosa Cells; Hormones; Influence; ovarian granulosa cells; Ovaries; Parameters; Plant Extracts - pharmacology; Progesterone; Progesterone - pharmacology; proliferation; Protected species; Salvia columbariae; Salvia hispanica; Swine; Testosterone; Xylene; Xylenes - pharmacology
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/rda.14355

The influence of the functional food plant chia (Salvia hispanica L.) on reproduction functions and its ability to prevent the negative effects of environmental contaminants has not yet been studied. Our study aimed to examine the effect of chia seed extract alone and in combination with xylene on the markers of proliferation, apoptosis and hormones release by cultured bovine and porcine ovarian granulosa cells. The extract of chia reduced all of the measured parameters in bovine and porcine ovarian cells but had no effect on the proliferation of porcine cells. Xylene, stimulated proliferation and IGF‐I release and inhibited the release of progesterone and testosterone but not apoptosis of bovine granulosa cells. It promoted proliferation, apoptosis and progesterone output by porcine cells. Chia mitigated the stimulatory effect of xylene on proliferation but not on other parameters in both species. The present results are the first demonstration of a direct effect of chia on basic ovarian cell functions. They confirmed a direct influence of xylene on these functions and found a similar stimulatory action of xylene on bovine and porcine ovarian cell proliferation. The present observations demonstrated species‐specific differences in the characteristics of xylene influences on ovarian cell apoptosis and secretory activity. Finally, the present results indicate that chia can be a natural protector against the proliferation‐stimulating effects of xylene on ovarian cells in both species.