Insulin-like growth factor II, a marker gene for determining the optimum dietary protein level in clownfish Amphiprion ocellaris

• Published in: Fish physiology and biochemistry 2024-02, Vol.50 (1), p.171-182
• Main Authors: Marudhupandi, Thangapandi, Dhayanithi, Nagarajan Balachandran, Jeyaprakashsabari, Sridharan, Deepa, Sugatha Dhas, Ajith Kumar, Thipramalai Thangappan, Sarkar, Uttam Kumar
• Format: Article
• Language: English
• Subjects: Amino acids; Amphiprion ocellaris; Animal Anatomy; Animal Biochemistry; Animal Physiology; Biomedical and Life Sciences; Body weight; Body weight gain; Conversion ratio; Feed conversion; Fish; Food conversion; Freshwater & Marine Ecology; Gene expression; Growth factors; Growth rate; Histology; In vivo methods and tests; Insulin; Insulin-like growth factor II; Insulin-like growth factors; Juveniles; Larvae; Life Sciences; Marine fishes; Minors; Morphology; Optimization; Proteins; Zoology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-023-01189-2

In vivo study was conducted to determine the role of insulin-like growth factors (IGF-II) related to dietary protein utilization. For this early juvenile stage of marine false clownfish Amphiprion ocellaris , 300 numbers of 20-day-old larvae (initial body weight of 18.2±0.027 mg/fish) were used as an experimental animal. Animals were fed for 12 weeks with different dietary protein levels (35, 40, 45, 50, and 55%), using Spirulina maxima as a major protein source. Proximate compositions and amino acid profile of the formulated diet were analyzed by the standard methods. Eventually, significant ( P