Particulate Standard Establishment for Absolute Quantification of Nanoparticles by LA-ICP-MS

The development of nanotechnology has transformed many cutting-edge studies related to single-molecule analysis into nanoparticle (NP) detection with a single-NP sensitivity and ultrahigh resolution. While laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been successful in...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2023-04, Vol.95 (15), p.6391-6398
Main Authors: Luo, Wenhe, Dong, Fengliang, Wang, Meng, Li, Tao, Wang, Yun, Dai, Wanqin, Zhang, Junzhe, Jiao, Chunlei, Song, Zhuda, Shen, Jiaqi, Ma, Yuhui, Ding, Yayun, Yang, Fang, Zhang, Zhiyong, He, Xiao
Format: Article
Language:English
Subjects:
Ablation
Calibration
Chemistry
Deep learning
Inductively coupled plasma mass spectrometry
Laser ablation
Laser Therapy
Lasers
Mass spectrometry
Mass Spectrometry - methods
Mass spectroscopy
Nanoparticles
Nanotechnology
Spectrum Analysis
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Summary:The development of nanotechnology has transformed many cutting-edge studies related to single-molecule analysis into nanoparticle (NP) detection with a single-NP sensitivity and ultrahigh resolution. While laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been successful in quantifying and tracking NPs, its quantitative calibration remains a major challenge due to the lack of suitable standards and the uncertain matrix effects. Herein, we frame a new approach to prepare quantitative standards via precise synthesis of NPs, nanoscale characterization, on-demand NP distribution, and deep learning-assisted NP counting. Gold NP standards were prepared to cover the mass range from sub-femtogram to picogram levels with sufficient accuracy and precision, thus establishing an unambiguous relationship between the sampled NP number in each ablation and the corresponding mass spectral signal. Our strategy facilitated for the first time the study of the factors affecting particulate sample capture and signal transductions in LA-ICP-MS analysis and culminated in the development of an LA-ICP-MS-based method for absolute NP quantification with single-NP sensitivity and single-cell quantification capability. The achievements would herald the emergence of new frontiers cut across a spectrum of toxicological and diagnostic issues related to NP quantification.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.3c00028