Screening of New Zealand native white-rot isolates for PCP degradation

From a pool of 367 white-rot fungi native to New Zealand (over 77 genera), isolates were screened for their bioremediation potential of pentachlorophenol (PCP). Fungi were tested for their ligninolytic activity (Poly R-478,367 isolates; wood decay, 235 isolates), tolerance to temperature (261 isolat...

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Bibliographic Details
Published in:Bioremediation journal 2003-06, Vol.7 (2), p.119-128
Main Authors: WALTER, Monika, GUTHRIE, Jenness M, SIVAKUMARAN, Siva, PARKER, Emily, SLADE, Ann, MCNAUGHTON, Don, BOYD-WILSON, Kirsty S. H
Format: Article
Language:English
Subjects:
Biodegradation of pollutants
Biological and medical sciences
Bioremediation
Biotechnology
Environment and pollution
Enzymes
Experiments
Fundamental and applied biological sciences. Psychology
Fungi
Industrial applications and implications. Economical aspects
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Summary:From a pool of 367 white-rot fungi native to New Zealand (over 77 genera), isolates were screened for their bioremediation potential of pentachlorophenol (PCP). Fungi were tested for their ligninolytic activity (Poly R-478,367 isolates; wood decay, 235 isolates), tolerance to temperature (261 isolates), resistance to PCP (253 isolates), and PCP degradation potential plus laccase expression (20 isolates). Of the isolates tested, 26% showed a discolouration in the polymeric dye assay, but all caused wood decay (5 to 169 mm) on willow cuttings. In the temperature tolerance tests, all isolates survived incubation from 0 to 30°C, however, 18% and 40% did not survive incubation at 35 and 40°C, respectively. In the PCP resistance tests, 23 isolates (9%) were able to grow on 200 mg/L PCP amended agar, of which 20 isolates were further studied for laccase expression and PCP degradation in vitro. All 20 isolates reduced (P < 0.05) PCP in the liquid fraction in the absence or presence of laccase and five of the isolates produced no detectable levels of PCP. None of the screening tests were predictive for PCP degradation in vitro. The requirements to build a database to select a superior white-rot fungal isolates for bioremediation is discussed. [PUBLICATION ABSTRACT] Keywords: bioremediation potential, Trametes versicolor, wood decay, polymeric dye-assay, PCP resistance, PCP degradation, laccase.
ISSN:1088-9868
1547-6529
DOI:10.1080/10889860390246150