Coupling Bifunctional Nanozyme-Mediated Catalytic Signal Amplification and Label-Free SERS with Immunoassays for Ultrasensitive Detection of Pathogens in Milk Samples

Rapid and sensitive detection of foodborne bacteria is of great significance in guaranteeing food safety and preventing foodborne diseases. A bifunctional Au@Pt core–shell nanozyme with excellent catalytic properties and high surface-enhanced Raman scattering (SERS) activity was developed for the hi...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2023-04, Vol.95 (15), p.6417-6424
Main Authors: Li, Zhihao, Hu, Jiao, Zhan, Yifang, Shao, Zhiyong, Gao, Mengyue, Yao, Qi, Li, Zheng, Sun, Shaowen, Wang, Lihua
Format: Article
Language:English
Subjects:
Amplification
Analytical chemistry
Animals
Biosensing Techniques
Biosensors
Chemistry
Coupling (molecular)
Food Safety
Foodborne Diseases
Foodborne pathogens
Gold
Gold - chemistry
Humans
Immunoassay
Immunoassays
Metal Nanoparticles - chemistry
Milk
Pathogens
Platinum
Raman spectra
Salmonella Typhi
Sensitivity
Spectrum Analysis, Raman
Substrates
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Summary:Rapid and sensitive detection of foodborne bacteria is of great significance in guaranteeing food safety and preventing foodborne diseases. A bifunctional Au@Pt core–shell nanozyme with excellent catalytic properties and high surface-enhanced Raman scattering (SERS) activity was developed for the highly sensitive detection of Salmonella typhimurium based on a label-free SERS strategy. The ultrathin Pt shell (about 1 nm) can catalyze Raman-inactive molecules into Raman-active reporters, greatly amplifying the amount of signal molecules. Moreover, the Au core serves as an active SERS substrate to enhance the signal of reporter molecules, further significantly improving the detection sensitivity. Benefiting from the excellent properties, such a bifunctional Au@Pt nanozyme was integrated with a magnetic immunoassay to construct a label-free SERS platform for the highly sensitive detection of S. typhi with a low detection limit of 10 CFU mL–1. Also, the Au@Pt-based SERS platform exhibited excellent selectivity and was successfully utilized for the detection of S. typhi in milk samples by a portable Raman spectrometer. Our demonstration of the bifunctional nanozyme-based SERS strategy provides an efficient pathway to improve the sensitivity of label-free SERS detection of pathogens and holds great promise in food safety, environmental analysis, and other biosensing fields.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.3c00251